BPS2024 Full Program & Abstracts

Exhibitor Presentations Rooms 103C and 113C, Pennsylvania Convention Center

Room 103C: Sunday, February 11 9:30 am – 11:00 am Bruker The Bruker Vutara VXL: Superresolution Microscopy Multiplexed and in 3D The Bruker Vutara VXL is a dedicated single-molecule localization (SMLM) super-resolution microscope. Historically, the optical resolu tion limit of fluorescence microscopy constrained the study of struc tures smaller than 300 nm. The Vutara VXL transcends this barrier, offering an optical resolution of 20 nm and beyond, and allows the unraveling of biological enigmas that necessitate specific labeling akin to fluorescence microscopy but demand a resolution surpassing that of diffraction-limited microscopy. One of the distinguishing features of the Vutara VXL is its proprietary bi-plane technology. This extends the traditional 2D fitting into the third dimension, enabling the imaging of thick cell cultures and tissue slices. The Vutara VXL does not require TIRF illumination. The Vutara VXL employs a variety of techniques to achieve super-reso lution. These include STORM, PALM, dSTORM, and DNA-PAINT. These techniques involve the modulation of dye molecule emissions and the transient binding of oligonucleotides. The result is a system capable of imaging, resolving, and quantifying cellular structures, molecular machines, proteins, RNA, and chromosomal structures with unprec edented detail. In addition to its super-resolution and 3D imaging capabilities, the Vutara VXL stands out for its multiplexing capabilities. It is equipped with two cameras for simultaneous multi-color imaging, allowing for the concurrent observation of multiple sub-cellular components. Furthermore, it integrates with the PlexFlo fluidics platform, enabling multiplexed experiments. This integration facilitates the simultaneous investigation of multiple biological phenomena, enhancing the effi ciency and scope of research. In this presentation, we will discuss how the Bruker Vutara VXL com pact bench-top system brings the power of SMLM into your lab. Speaker Winfried Wiegraebe, Product Manager Superresolution Microscopy, Bruker

11:30 am – 1:00 pm Carl Zeiss Microscopy LLC ZEISS Dynamics Profiler – Push Fluorescence Correlation Spectroscopy to a New Level The ZEISS Dynamics Profiler for Airyscan 2 offers an innovative approach to studying molecular dynamics in living samples, expanding upon traditional fluorescence correlation spectroscopy methods. With remarkably easy access to molecular dynamics, researchers can gain deeper insights into complex biological systems. The unique design of the Airyscan 2 detector enables the Dynamics Profiler to provide new discoveries through asymmetric diffusion and flow analyses; hence, providing a comprehensive view of molecular dynamics. With the abil ity to study a wide range of biological samples, the Dynamics Profiler is a powerful tool for researchers in the life sciences seeking to advance their understanding of dynamic biological processes. Speaker Samantha Fore, Life Sciences Product Marketing Manager Laser Scanning Microscopy, ZEISS Research Microscopy Solutions North America 1:30 pm – 3:00 pm Mad City Labs Inc Looking to the Future – Enabling Single Molecule Methods for Improved Health Outcomes This session features researchers employing single molecule methods aimed at improving medical diagnosis or health outcomes. The empha sis is on the challenges facing scientists to develop improved methods and sensors to achieve these outcomes. Interfacing Coherent Qubits with Biological Targets Speaker: Uri Zvi, Pritzker School of Molecular Engineering, University of Chicago Prof. Peter Maurer’s lab at the University of Chicago investigates new quantum sensing techniques and their application to probe physical properties of biological processes with nanoscale resolution. The sci entific challenges our lab addresses fall roughly into two distinct yet synergetic areas: (1) What are the sensitivity limits of nanoscale quan tum sensors in a noisy environment and can we engineer qubit sensors and sensing protocols that overcome these limitations? (2) How can we interface these qubit sensors with biological systems and what are the specific biological questions that we can address with quantum sens ing? Single Molecule Pharmacology Reveals the Kinetic Mechanism of Action of Splicing Modulators Speaker: Aaron Hoskins, Wasson Professor of Biochemistry, University of Wisconsin-Madison Therapeutics targeting pre-mRNA splicing are a promising avenue to treat human diseases, including spinal muscular atrophy and Huntington’s disease. Here, we use single molecule colocalization spec troscopy (CoSMoS) to directly measure modulation of 5' splice site rec ognition by human U1 snRNP. By leveraging large single molecule data sets with >50,000 immobilized molecules and >33 million video frames,

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