Biophysical Society 2020 Annual Meeting

Optical Microscopy and Superresolution Imaging III (Boards B533 - B542) 2263-Pos Board B533 Travel Awardee A COMPARISON OF HISTO-CHEMICAL AND HISTO-MAGNETIC DETECTION OF IRON. Kevin J. Walsh , Stavan Shah, Ping Wei, Samuel Oberdick, Dana McTigue, Gunjan Agarwal 2264-Pos Board B534 INTERPLAY OF RADIATIVE AND NON RADIATIVE RATE CONSTANTS IN THE PHOTOPHYSICS OF FLUORESCENT PROTEINS. Srijit Mukherjee 2265-Pos Board B535 THE VASCULAR BARRIER REGULATES CARDIAC NANODOMAINS: IMPLI- CATIONS FOR THE GENESIS OF ATRIAL FIBRILLATION. Louisa Mezache , Heather Struckman, Anna Phillips, Stephen Baine, Amara Greer-Short, Sandor Gyorke, Przemyslaw Radwanski, Thomas J. Hund, Rengasayee Veeraraghavan 2266-Pos Board B536 PUMPLESS MICROFLUIDIC SYSTEM FOR BONE MARROW NICHE-ON-A- CHIP IN VITRO MODELLING AND MULTIPHOTON IMAGING IN LEUKE- MIA. Giulia Borile , Giulia Borella, Camille Charoy, Andrea Filippi, Filippo Romanato, Martina Pigazzi, Kurt Anderson 2267-Pos Board B537 EXCLUSION OF RNA-ASSOCIATED PROTEINS FROM THE CELL CORTEX OBSERVED BY DUAL COLOR Z-SCAN FLUORESCENCE MICROSCOPY.  Siddarth Reddy Karuka , Isaac Angert, John Kohler, Louis M. Mansky, Joachim D. Mueller 2268-Pos Board B538 AN ULTRA-SENSITIVE IMMUNOHISTOCHEMICAL (IHC) IMAGING METHOD FOR LOW-ABUNDANT TARGETS DETECTION. Haiyan Wu, Shu Kan, Deven Patel, Qin Zhao , Pengfei Dong, Liu Jixiang, Jinfang Liao, Zhenjun Diwu 2269-Pos Board B539 REAL-TIME POINT SPREAD FUNCTION ENGINEERING FOR ISCAT.  Vivien Walter , Mark I. Wallace 2270-Pos Board B540 LARGE-SCALE SPECIES-SPECIFIC MICROBIAL IDENTIFICATION BY FLUORES- CENCE IN SITU HYBRIDIZATION. Sungho Kim , Jae-Kyeong Im, Seungmin Yun, Hwasooo Koh, Donghoon Kang, Taejoon Kwon, Hajin Kim 2271-Pos Board B541 SIMULTANEOUS IMAGING OF INSULIN VESICLE DYNAMICS AND CALCIUM ACTIVITY IN LIVE INTACT MOUSE ISLETS BY DISPIM. Xue Wen Ng , Michael R. DiGruccio, Tomasz S. Tkaczyk, David W. Piston 2272-Pos Board B542 POINT SPREAD FUNCTION ENGINEERING TO MAP 3D PARTICLE MO- TION. Keith Bonin , Sudhakar Prasad, Paul Kefer, George M. Holzwarth, Pierre-Alexandre Vidi Single-Molecule Spectroscopy I (Boards B543 - B559) 2273-Pos Board B543 SINGLE-MOLECULE DYNAMICS OF THE HUMAN MITOCHONDRIAL RNA POLYMERASE PREINITIATION COMPLEX. Rory F. Cunnison , Emily Teece, Jonathan Grimm, Luke D. Lavis, Dmitry Temiakov, Yaroslav Morozov, Andrey G. Revyakin 2274-Pos Board B544 THE ROTARY MOTOR OF LIFE: SINGLE-MOLECULE IMAGING AND MO- LECULAR DYNAMICS SIMULATION OF F1-ATPASE. Nathan Suiter , Jason Portillo, Matthew A. Anderson

2275-Pos Board B545 EGFR MEMBRANE DYNAMICS AND ORGANIZATION INVESTIGATED BY CAMERA-BASED MULTI-PARAMETER FLUORESCENCE IMAGING WITH HIGH SPATIOTEMPORAL RESOLUTION. Thorsten Wohland , Jagadish San- karan, Harikrushnan Balasubramanian 2276-Pos Board B546 INSIDE-OUT REGULATION OF CADHERIN ADHESION. Ramesh Koirala , Andrew V. Priest, Soichiro Yamada, Martijn Gloerich, Sanjeevi Sivasankar 2277-Pos Board B547 RAISING THE BAR ON SINGLE-MOLECULE BIOPHYSICS: DNA/RNA SEC- ONDARY/TERTIARY FOLDING USING EXTREME PRESSURE AS A CONTROL VARIABLE. Hsuan-Lei Sung , David J. Nesbitt 2278-Pos Board B548 Board B549 Travel Awardee CPG METHYLATION DETECTION WITH SINGLE-MOLECULE RECOGNITION THROUGH EQUILIBRIUM POISSON SAMPLING. Liuhan Dai , Alexander Johnson-Buck, Muneesh Tewari, Nils G. Walter 2280-Pos Board B550 SPCAS9 DISPLAYS BIASED ONE-DIMENSIONAL DIFFUSION ON DSDNA TO SEARCH FOR A TARGET. Chunlai Chen , Mengyi Yang 2281-Pos Board B551 SPECTRAL ANALYSIS OF A FAST BIOMOLECULAR TRANSITION IN MAG- NETIC TWEEZERS MEASUREMENTS. Sebastian Belau , Ralf Seidel 2282-Pos Board B552 ANTIBODY BINDING BACTERIA SAMPLE THEIR ENVIRONMENT THROUGH A SECOND BINDING SITE, WHICH CAN ACT AS A FORCE-SENSOR UNDER MECHANICAL SHEER. Narayan Dahal, Joel Nowitzke, Annie Eis, Ionel Popa 2283-Pos Board B553 COMBINED SINGLE-MOLECULE FRET AND SINGLE-CHANNEL RECORDING TO LINK ION CHANNEL CONFORMATION AND FUNCTION. Steven Vanuytsel , Christopher L. Parperis, Mark I. Wallace 2284-Pos Board B554 RESOLUTION OF ANGSTROM-SCALE PROTEIN-CONFORMATIONAL CHANGES IN THE REGULATORY DOMAIN OF A K + CHANNEL BY ANALYZ- ING FLUORESCENCE ANISOTROPY.  John H. Lewis , Zhe Lu 2285-Pos Board B555 MULTIVALENT EFFECTS ON INTERACTIONS BETWEEN THE LIGAND AND CELL-SURFACE RECEPTORS PROBED BY A BINDING FORCE SPECTROSCO- PY. Lina A. Alhalhooly , Matthew Confeld, Yongki Choi, Sanku Mallik 2286-Pos Board B556 USING SINGLE-MOLECULE SPECTROSCOPY TO DISSECT THE HEPATITIS C VIRUS NUCLEOCAPSID ASSEMBLY PATHWAY. Saptaswa Sen, Shamal Ungawel Durayalage, Erik D. Holmstrom 2287-Pos Board B557 A STUDY OF TRANSCRIPTIONAL ACTIVATION BY THE TRANSCRIPTION FACTOR GAL4 IN SACCHAROMYCES CEREVISIAE BY 3D ORBITAL TRACK- ING AND IN VIVO RNA LABELLING. Abigail Figueroa, Iris L. Torres , Julianna Goelzer, Michael Pool, Tineke Lenstra, Matthew L. Ferguson 2288-Pos Board B558 Travel Awardee RNA TRAFFICKING BETWEEN MEMBRANELESS ORGANELLES AT SINGLE- MOLECULE RESOLUTION IN LIVE CELLS. Guoming Gao , Ameya P. Jalihal, Andreas Schmidt, Nils G. Walter 2289-Pos Board B559 SINGLE-MOLECULE MEASUREMENTS TO CAPTURE THE DISTRIBUTION OF CONFORMATIONAL AND DIMERIC STATES OF THE CYTOSOLIC PROTEIN CRAF IN LIVE CELLS. Kenji Okamoto , Kayo Hibino, Yasushi Sako SINGLE-MOLECULE G-QUADRUPLEX NANOPORE ASSAY.  Filip N. Boskovic , Jinbo Zhu, Kaikai Chen, Ulrich F. Keyser 2279-Pos

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