Biophysical Society 2020 Annual Meeting

Exhibitor Presentation Mizar Imaging 9:30 am - 11:00 am, Room 33A

aggregates form, properties of conjugates such as determination of the mass of pegylation or many other conjugates relative to the mass of the protein, protein conformation and many others. One of the properties of light scattering that differentiate it from other techniques that give similar data is the ability for the experiments to be done in solution. With no labeling, fixing of detection agents to solid surfaces or drying of the material to be analyzed you get a real picture of the properties in a given solution. In this presentation we will discuss the recent advances in HPLC, field flow fractionation (FFF) and composition gradient (CG) coupled with multi-angle light scattering (MALS).  The use of HPLC has expanded beyond size exclusion chromatography to include ion-exchange, reversed phase and hydrophobic interaction chromatography that enables the assessment of other properties and various types of molecules such as antibody drug conjugates. FFF-MALS is a gentle separation technique that allows for the separation of a wide range of particle sizes in a single channel with low shear. It is done entirely in a liquid stream and is well suited to utilizing the same separation buffer in which the molecules have been formulated, eliminating the worry that the elution buffer may be af- fecting the molecule in some way. With CG-MALS the user is able to study protein interaction with other molecules of interest again all in solution and label free. We invite you to join us in this discussion of the newest uses to discover how they might apply to the next breakthrough in your research. Speaker Kevin McCowen, Regional Manager, Wyatt Technology Symposium Chair Brian Baker, University of Notre Dame No Abstract 10:45 am A SYSTEMS APPROACH TO ENGINEERED IMMUNITY - FROMMOLECULES AND CELLS TO PATIENTS.  Krishnendu Roy 67-Symp 11:15 am HOW TO HIT HIV WHERE IT HURTS.  Arup Chakraborty 68-Symp 11:45 am MULTI-SCALE COMPUTATIONAL MODEL OF IMMUNE CELL ACTIVATION IN CANCER.  Stacey D. Finley No Abstract 12:15 pm DEMYSTIFYING CROSS-REACTIVITY IN CELLULAR IMMUNITY.  Brian M. Baker Symposium Mapping the Immune System 10:45 am - 12:45 pm, Ballroom 20A

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Tilt – A New Angle on Light Sheet Imaging Mizar Imaging is proud to introduce the Tilt, the first light sheet imaging system that is a simple add-on to most inverted microscopes. The key benefit of light sheet imaging is significantly reducing the photobleach- ing and phototoxicity of your sample and the Tilt excels at this. When imaging with the Tilt, cells can be kept alive for hours and even days. This is aided by an optional incubation chamber for the Tilt, which allows for precise control of temperature (heating and cooling available), CO2 and humidity. When installed on your microscope, the Tilt does not interfere with any existing modalities so you can easily add the Tilt to an existing TIRF or spinning disc confocal microscope system to add the ability to do long-term, live-cell imaging with the lowest possible photobleaching and phototoxicity. The Tilt is well suited to image both larger organisms, such as C. elegans , Drosophila , zebra fish and other similar model organisms as well imaging high-resolution intracellular dynamics inside single cells. This remarkable diversity is realized because the Tilt can work with any objective on your microscope – from 20x through 150x. This makes the Tilt the only light sheet imaging system that can use high NA/high magnification objectives such as high resolution 60x and 100x objectives. There is no limit to what you can do with the Tilt. The Tilt light sheet imaging system is the ideal solution for long-term live- cell imaging of a wide array of samples with the added benefit of being a simple, low cost add-on to an existing inverted microscope. Speaker Paul Maddox, Founder & President, Mizar Imaging Exhibits 10:00 am - 5:00 pm, Exhibit Hall Coffee Break 10:15 am - 11:00 am, Exhibit Hall Career Development Center Workshop Green Cards for Scientific Researchers: How to Win Your EB-1A/NIW Case! with Getson & Schatz, PC 10:30 am - 11:30 am, Room 26A Brian Getson is a leading U.S. immigration lawyer who represents scientific researchers in applying for green cards in the EB-1A, EB-1B and NIW categories. Learn about the U.S. immigration process and how to maximize your chances of immigration success during this workshop. He will answer questions and provide free legal consultations after the presentation and throughout BPS 2020. Exhibitor Presentation Wyatt Technology 10:30 am - 12:00 pm, Room 33C Recent Advances in Light Scattering and Related Techniques Historically light scattering detection has been seen as a tool to assess molecular weight and aggregation. Throughout its existence the utility of this method to assess additional properties of proteins has expanded significantly. Today it’s uniquely positioned to give information about how

Cytoskeleton and Motility 10:45 am - 12:45 pm, Ballroom 20D

Chair Joseph Falke, University of Colorado Boulder 69-Symp 10:45 am

HOW DOES THE ACTIN CYTOSKELETON REGULATE DISTRIBUTION AND DIFFUSION OF MEMBRANE COMPONENTS?  Barbara Baird , David Ho- lowka 70-Symp 11:15 am REGULATION OF ACTIN AND MEMBRANE DYNAMICS BY CLASS I MYO- SINS. Mira Krendel 71-Symp 11:45 am MECHANOCHEMICAL CIRCUITS IN THE CYTOPLASM. Margaret Gardel

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