Biophysical Society 2020 Annual Meeting

355-Pos Board B186 UNCOVERING THE MOLECULAR BASIS FOR SEQUENCE-DEPENDENT TRANSLOCATION IN A SUPERFAMILY 2 HELICASE USING HIGH-RESO- LUTION NANOPORE TWEEZERS.  Jonathan M. Craig , Sinduja K. Marx, Andrew H. Laszlo, Ian C. Nova, Hwanhee C. Kim, Jesse R. Huang, Sarah J. Abell, Jens H. Gundlach 356-Pos Board B187 DIRECT MEASUREMENT OF STEPPING DYNAMICS OF E. COLI UVRD HELI- CASE. Sean P. Carney , Kevin D. Whitley, Wen Ma, Haifeng Jia, Timothy M. Lohman, Zaida Luthey-Schulten, Yann R. Chemla 357-Pos Board B188 MOLECULAR MECHANISM OF CONFORMATIONAL SWITCHING THAT REGULATES HELICASE FUNCTION. Wen Ma , Sean Carney, Yann R. Chemla, Zaida Luthey-Schulten, J. AndrewMcCammon 358-Pos Board B189 THE NUCLEASE DOMAIN OF RECBCD INFLUENCES DNA BINDING AND HELICASE ACTIVITY. Nicole T. Fazio , Linxuan Hao, Timothy M. Lohman 359-Pos Board B190 SV40 LARGE T ANTIGEN HELICASE UNWINDS DOUBLE-STRANDED DNA IN SINGLE-NUCLEOTIDE STEPS AS REVEALED BY NANOPORE TWEE- ZERS. Christopher A. Thomas , Jonathan M. Craig, Andrew H. Laszlo, Ian C. Nova, Sherry Xie, Jesse R. Huang, Hwanhee C. Kim, Sarah J. Abell, Hasan Yardimci, Jens H. Gundlach 360-Pos Board B191 ROLE OF ATP IN ALLOSTERIC REGULATION OF THE DNA CLEAVAGE PRO- CESS IN YEAST TOPOISOMERASE IIA. Stefania Evoli , Jeffery M. Wereszc- zynski 361-Pos Board B192 DOMAIN RIGIDITY MODULATES THE CATALYTIC ACTIVITY OF E. COLI TYPE IA DNA TOPOISOMERASES. Yeonee Seol , Yuk-Ching Tse Dinh, Keir C. Neu- man 362-Pos Board B193 DISCOVERY OF NOVEL DNA GYRASE INHIBITORS AGAINST SALMONELLA TYPHI USING STRUCTURE BASED DRUG DELIVERY APPROACH. Arti Kapil , Priyanka Sharma, Punit Kaur, Manoj Kumar, Sushila Dahiya, Seema Sood, Bimal Das 363-Pos Board B194 INVESTIGATING THE ROLE OF MSH4-MSH5 ATPASE ACTIVITY DURING HOMOLOGOUS RECOMBINATION. Zane Lombardo , Sudipta Lahiri, Bharat Lakhani, Manju M. Hingorani, David L. Beveridge, Ishita Mukerji 364-Pos Board B195 SINGLE-MOLECULE MAGNETIC TWEEZERS CHARACTERIZATION OF MTERF1 AS A DIRECTIONAL ROADBLOCK. Eugeniu Ostrofet , Flavia Stal Papini, Britney Johnson, Jamie J. Arnold, Craig E. Cameron, David Dulin 365-Pos Board B196 HIV RESTRICTION FACTOR APOBEC3G BINDS SINGLE STRANDED DNA IN MULTIPLE CONFORMATIONS WHILE SEARCHING FOR TARGET DEAMI- NATION SITES. Michael Morse , M. Nabuan Naufer, Yuqing Feng, Linda Chelico, Ioulia Rouzina, Mark C. Williams 366-Pos Board B197 SINGLE-MOLECULE STUDIES OF HIV-1 GAG ASSEMBLY. Raymond F. Pauszek , Arishma Singh, Jonathan Kitzrow, Shuohui Liu, Karin Musier- Forsyth, David P. Millar 367-Pos Board B198 PROBING THE BINDING MECHANISM OF DNA POLYMERASE KAPPA TO DNA USING OPTICAL TWEEZERS.  Joshua Watts , Samer Lone, Thayaparan Paramanathan

368-Pos Board B199 INTRINSIC DISORDER DIRECTS TWO DISTINCT DIMERS OF THE MASTER TRANSCRIPTION FACTOR PU.1. Suela Xhani, Sangchoon Lee, Markus W. Germann, Gregory M. Poon 369-Pos Board B200 POSITIVE SUPERCOILING AHEAD OF RNA POLYMERASE AIDS EXIT FROM PROTEIN-MEDIATED LOOPS. Wenxuan Xu , Yan Yan, David Dunlap, Laura Finzi 370-Pos Board B201 TRANSCRIPTIONAL OBSTACLES MODIFY REPETITIVE ELONGATION.  Yan Yan , Wenxuan Xu, Jin Qian, Irina Artsimovitch, David Dunlap, Laura Finzi 371-Pos Board B202 STRUCTURAL BASIS OF 7SK RNA 5’-GAMMA-PHOSPHATE METHYLATION AND RETENTION BY MEPCE. Yuan Yang , Catherine D. Eichhorn, Yaqiang Wang, Duilio Cascio, Juli Feigon 372-Pos Board B203 A HIGH-THROUGHPUT PLATFORM CHARACTERIZES FUNCTIONAL EFFECTS OF TRANSCRIPTION FACTOR MUTATIONS. Arjun K. Aditham , Nicole V. DelRosso, Polly Fordyce 373-Pos Board B204 CHARACTERIZATION OF THE NOVEL DNA BINDING ACTIVITY OF THE BRG1 AT-HOOK-BROMODOMAIN.  Julio C. Sanchez , Liyang Zhang, Miles Pufall, Catherine A. Musselman 374-Pos Board B205 INVESTIGATING THE NOVEL NUCLEIC ACID BINDING ACTIVITY OF POLY- BROMO-1 BROMODOMAINS. Saumya M. De Silva , Nicholas J. Schnicker, Catherine A. Musselman 375-Pos Board B206 REGULATION OF VIRAL PACKAGING MOTORS GRIP ON DNA AND DIS- COVERY OF A DNA “END-CLAMP” MECHANISM. Mariam Ordyan, Mounir Fizari, Jo-fan Chien, Brandon Rawson, Douglas E. Smith 376-Pos Board B207 CHARACTERISTIC INTERACTIONS BETWEEN BRCA2 AND G-QUADRUPLEX STRUCTURES FOR TELOMERE MAINTENANCE. Keewon Sung , Junyeop Lee, So Young Joo, Hyunsook Lee, Seong Keun Kim 377-Pos Board B208 IN SILICO STUDIES ON FUNCTIONAL SIGNIFICANCE OF MULTIPLE BINDING CONFIGURATIONS OF BACTERIAL NUCLEOID ASSOCIATED PROTEIN-DNA ASSEMBLIES. Min-Yeh Tsai , Weihua Zheng, Mingchen Chen, Peter G. Board B209 CHROMATIN FOLDING UNDER DIFFERENT NUCLEAR CONFINE- MENT. Samira Mali , Alan Perez-Rathke, Qiu Sun, Gamze Gürsoy, Jie Liang 379-Pos Board B210 Travel Awardee COARSE-GRAINED MODELING OF PRC2-MEDIATED INTER-NUCLEOSOMAL INTERACTIONS. Xingcheng Lin , Rachel Leicher, Eva Ge, Matthew J. Reyn- olds, Thomas Walz, TomMuir, Shixin Liu, Bin Zhang 380-Pos Board B211 UNDERSTANDING PROTEIN-DNA RECOGNITION IN THE CONTEXT OF MULTI-SCALE GENOME ORGANIZATION. Remo Rohs 381-Pos Board B212 FLUORESCENT PROTEIN AS A DNA STAINING DYE. Kyubong Jo Wolynes 378-Pos

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