Biophysical Society 2020 Daily Schedule

Exhibitor Presentations Rooms 33A 33C, San Diego Convention Center

Room 33A: Sunday, February 16 9:30 AM – 11:00 AM Mizar Imaging Tilt – A New Angle on Light Sheet Imaging

11:30 AM – 1:00 PM NanoSurface Biomedical Recreating the Extracellular Matrix in a Dish

Cells in the body use a variety of cues (e.g. structural, mechanical, electrical, and chemical) from the extracellular matrix (ECM) to develop and mature physiologically. These influential cues help regulate a broad spectrum of processes such as cell signaling, division, and differentiation. Many in vitro platforms seek to incorporate these cues into the cell’s microenvironment, but often fail, suffering from lack of reproducibility and incompatibility with other well-established end-point assays. Here, we demonstrate biomimetic in vitro platforms capable of reliably repro- ducing these essential ECM cues. These platforms markedly improve the structural and functional development of a variety of cell types, including stem cells, cardiomyocytes, muscle cells, and many more. Specifically, we show how NanoSurface Plates and Cytostretcher Cell- stretching Instruments can be utilized individually or collectively to study various model systems. The effects of cell-nanotopography interactions on adhesion, signaling, polarity, and migration across many applications such as human epithelia, cardiovascular function, and cancer biology are highlighted. Further, we describe how the differentiation of stem cells can be enhanced by providing a more biomimetic culture environment, with a particular focus on iPSC-derived cardiomyocytes and skeletal muscle cells. Speaker Hamed Ghazizadeh, Product Manager, NanoSurface Biomedical Multiplex Mode for the LSM 9 Series with Airyscan 2: Fast and Gentle Confocal Superresolution in Large Volumes The LSM 9 family with Airyscan 2 from ZEISS provides more options to enable the perfect balance of speed and resolution for today’s confocal- imaging needs. The new Multiplex mode extends sensitive Airyscan imaging to larger model systems with low expression levels by increas- ing acquisition speeds even further. It extracts more spatial information; hence, multiple lines can be imaged in a single line scan. This allows for larger acquisition steps to improve image acquisition speeds and reduce the illumination dosage to the sample. This novel concept allows rapid volumetric imaging with unprecedented resolution beyond what is avail- able in traditional confocal systems today. Airyscan 2 provides new data handling concepts, providing 6.6 times smaller data sizes and 5 times faster image reconstruction times. Further, optimized real time acquisition strategies employed with the LSM 9 family enable faster scan speeds for Airyscan 2, allowing higher data throughput. Join this workshop and learn how the newest members of the ZEISS imaging portfolio, ZEISS LSM 9 series with Airyscan 2 can help you cap- ture dynamic processes in volumes and improve your imaging experi- ments in completely new ways. Speaker Renée Dalrymple, Product Marketing Manager-Laser Scanning Microscopy, Carl Zeiss Microscopy LLC 1:30 PM – 3:00 PM Carl Zeiss Microscopy LLC

Mizar Imaging is proud to introduce the Tilt, the first light sheet imaging system that is a simple add-on to most inverted microscopes. The key benefit of light sheet imaging is significantly reducing the photobleach- ing and phototoxicity of your sample and the Tilt excels at this. When imaging with the Tilt, cells can be kept alive for hours and even days. This is aided by an optional incubation chamber for the Tilt, which allows for precise control of temperature (heating and cooling available), CO2 and humidity. When installed on your microscope, the Tilt does not interfere with any existing modalities so you can easily add the Tilt to an existing TIRF or spinning disc confocal microscope system to add the ability to do long- term, live-cell imaging with the lowest possible photobleaching and phototoxicity. The Tilt is well suited to image both larger organisms, such as C. elegans, Drosophila, zebra fish and other similar model organisms as well imaging high-resolution intracellular dynamics inside single cells. This remark- able diversity is realized because the Tilt can work with any objective on your microscope – from 20x through 150x. This makes the Tilt the only light sheet imaging system that can use high NA/high magnification objectives such as high resolution 60x and 100x objectives. There is no limit to what you can do with the Tilt. The Tilt light sheet imaging system is the ideal solution for long-term live- cell imaging of a wide array of samples with the added benefit of being a simple, low cost add-on to an existing inverted microscope. Speaker Paul Maddox, Founder & President, Mizar Imaging

20