Biophysical Society 2020 Daily Schedule

Room 33A: Tuesday, February 18 9:30 am – 11:00 am Sophion Bioscience A/S

Room 33C: Sunday, February 16 10:30 AM – 12:00 PM Wyatt Technology

Characterization of the Rapidly Desensitizing α 7 Nicotinic Acetylcholine Receptor on the Qube, NaV1.1 Assays on Automated Electrophysiology Platforms and Developing NMDA Assays on the Qube System Successful ion channel drug discovery requires the integration of mul- tiple technologies and workflows. Sophion Bioscience is a leader in auto- mated patch clamp technology, providing medium to high throughput, automated patch clamp to the pharmaceutical industry and universities. The QPatch and Qube are fully automated patch clamp systems, execut- ing simultaneous 8, 16, 48 or 384 parallel patch clamp recordings in conjunction with computer controlled liquid handling and on-board cell handling. Sophion partners with other biotech companies to create robust, ion channel and electrophysiological workflows for drug devel- opment for ion channel targets. During this workshop, three industry speakers will provide insight into the use of these systems in the drug discovery process. Dr Sung Hoon Park will present Qube data to show the characterization of rapidly desensitizing α7 nicotinic acetylcholine receptor on the Qube. Next, Dr Shanti Amagasu from Amgen will pres- ent data from Amgen’s Nav1.1. work on automated electrophysiological platforms. Finally, Dr Abigail Marklew will present on the development of NMDA Assays on the Qube system. Speakers Sung Hoon Park, Field Application Scientist, Sophion Bioscience A/S Shanti Amagasu, Senior Scientist, Amgen Abigail Marklew, Scientist, Charles River Laboratories A New Imaging Camera Technology Featuring TDC In-Pixel Architecture for Simple Dynamic FLIM Imaging at Video Rates A new wide-field video rate TCSPC imaging camera from HORIBA Instruments will be introduced. This camera is a CMOS manufactured array of single photon avalanche diode (SPAD) detectors, with each detection “pixel” having its own time-to-digital converter (TDC). Thus each pixel is capable of measuring precise fluorescence decays in time- domain, and the entire camera is providing a complete fluorescence lifetime image map (FLIM) with each frame of the camera. This new technology is much faster than traditional scanning FLIMmodalities thus making it ideal for live cell FLIM dynamics. Speaker Cary Davies, Global Product Manager-Fluorescence Division, HORIBA Scientific 1:30 pm – 3:00 pm HORIBA Scientific

Recent Advances in Light Scattering and Related Techniques Historically light scattering detection has been seen as a tool to assess molecular weight and aggregation. Throughout its existence the utility of this method to assess additional properties of proteins has expanded significantly. Today it’s uniquely positioned to give information about how aggregates form, properties of conjugates such as determination of the mass of pegylation or many other conjugates relative to the mass of the protein, protein conformation and many others. One of the proper- ties of light scattering that differentiate it from other techniques that give similar data is the ability for the experiments to be done in solution. With no labeling, fixing of detection agents to solid surfaces or drying of the material to be analyzed you get a real picture of the properties in a given solution. In this presentation we will discuss the recent advances in HPLC, field flow fractionation (FFF) and composition gradient (CG) coupled with multi-angle light scattering (MALS). The use of HPLC has expand- ed beyond size exclusion chromatography to include ion-exchange, reversed phase and hydrophobic interaction chromatography that enables the assessment of other properties and various types of mole- cules such as antibody drug conjugates. FFF-MALS is a gentle separation technique that allows for the separation of a wide range of particle sizes in a single channel with low shear. It is done entirely in a liquid stream and is well suited to utilizing the same separation buffer in which the molecules have been formulated, eliminating the worry that the elution buffer may be affecting the molecule in some way. With CG-MALS the user is able to study protein interaction with other molecules of interest again all in solution and label free. We invite you to join us in this discussion of the newest uses to discover how they might apply to the next breakthrough in your research. Speaker Kevin McCowen, Regional Manager, Wyatt Technology For over 45 years, Sutter Instrument has been collaborating with researchers. During this period, there have been many technological evolutions in patch clamp electrophysiology, and Sutter has introduced many new product families, including pipette pullers, manipulators, light sources, wavelength switchers, specialized microscopes and, most recently, fully integrated patch clamp amplifier systems. At this pre- sentation, we will teach techniques, tips and tricks, and showcase new features, such as dynamic clamp capability. The IPA®, Double IPA® and new dPatch® Ultra-fast, Low-noise Integrated Patch Clamp Amplifiers and SutterPatch® Software are being used for a variety of common experiments, including characterization of ionic cur- rent and recording synaptic events in tissue slices. We will demonstrate how the SutterPatch Software’s online measurements and sophisticated control of experimental workflow can be used to aid real-time decision- making and eventually simplify analysis. 12:30 pm – 2:00 pm Sutter Instrument Scientists Empowering Scientists

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