Biophysical Society 59th Annual Meeting Program Guide

128-P lat 11:15 am STRUCTURAL POLYMORPHISM OF CAG RNA REPEATS INVESTIGATED BY SINGLE-MOLECULE MECHANICAL UNFOLDING. Pan T. Li , William Stephenson 129-P lat 11:30 am HOW FLAVIVIRUSES USE A UNIQUE ‘SLIPKNOT-LIKE’ STRUCTURE TO MECHANICALLY CONFOUND A CELLULAR EXONUCLEASE AND PRODUCE PATHOGENIC RNA. Erich G. Chapman, David A. Costantino, Jennifer L. Rabe, Stephanie L. Moon, Jay C. Nix, Jeffrey Wilusz, Jeffrey S. Kieft 130-P lat 11:45 am MAPPING LONG NON-CODING RNA STRUCTURES WITH FRAGMENTATION AND NEXT-GENERATION SEQUENCING. Jeffrey Vieregg , William Richter, Alex Ruthenburg 131-P lat 12:00 pm U2 SNRNA CONFORMATION IS REGULATED BY CUS2 TO FACILITATE DEAD-BOX PROTEIN LOADING. U. Sandy Tretbar, Aaron Hoskins 132-P lat 12:15 pm RNA FLEXIBILITY DEPENDS ON STRUCTURAL CONTEXT. Julie Sutton , Lois Pollack 133-P lat 12:30 pm SINGLE-MOLECULE STUDIES OF KISSING LOOP INTERACTIONS IN GUANINE RIBOSWITCH. Maumita Mandal 10:45 am –12:45 pm , R oom 309/310 Platform Membrane Pumps, Transporters, and Exchangers I Co-Chairs Gregory Verdon, Imperial College London, United Kingdom Joseph Mindell, NIH/NINDS 134-P lat 10:45 am STRUCTURE AND BIOCHEMICAL CHARACTERIZATION OF THE MAMMALIAN FRUCTOSE TRANSPORTER GLUT5. Gregory Verdon , Hae Joo Kang, Saba Abdul-Hussein, Abdul Aziz Qureshi, Michihiro Kasahara, So Iwata, Norimichi Nomura, David Drew 135-P lat 11:00 am THE CONFORMATIONAL DYNAMICS OF A SECONDARY MULTIDRUG TRANSPORTER ARE MODULATED BY THE LIPID BILAYER COMPOSITION. Chloé Martens 136-P lat 11:15 am THERMODYNAMIC FEATURES OF IIAGLC INHIBITION OF SUGAR SYMPORTERS. Hariharan Parmeswaran , Balasubramaniam Dhandayuthapani, Alan Peterkofsky, Ronald H. Kaback, Lan Guan 137-P lat 11:30 am TRANSPORT COUPLING STOICHIOMETRY DETERMINATION OF ELECTROGENIC SECONDARY TRANSPORTERS. Gabriel A. Fitzgerald , Christopher Mulligan, Joseph A. Mindell 138-P lat 11:45 am LIGAND-DEPENDENT CONFORMATIONAL CYCLE OF THE NA+/HYDANTOIN TRANSPORTER MHP1. Kelli Kazmier , Shruti Sharma, Shahidul M. Islam, Benoit Roux, Hassane S. Mchaourab 139-P lat 12:00 pm A KEY ROLE FOR TM5 IN THE NA + -COUPLED ALTERNATING- ACCESS MECHANISM REVEALED BY COMPUTATIONAL ANALYSIS OF THE MHST STRUCTURES. Zheng Li, Sebastian Stolzenberg , Matthias Quick, Lina Malinauskaite, Poul Nissen, Harel Weinstein, Jonathan A. Javitch, Lei Shi

10:45 am –12:45 pm , B allroom IV Platform Cytoskeletal Mechanics, Dynamics, Motility, and Myosins Co-Chairs Michael Greenberg, University of Pennsylvania Harvey Chin, Columbia University 118-P lat 10:45 am cpow travel awardee SITE-SPECIFIC CATION RELEASE DRIVES ACTIN FILAMENT SEVERING BY VERTEBRATE COFILIN. Hyeran Kang , Michael J. Bradley, Wenxiang Cao, Kaifeng Zhou, Elena E. Grintsevich, Alphée Michelot, Emil Reisler, Charles V. Sindelar, Mark Hochstrasser, Enrique M. De La Cruz 119-P lat 11:00 am HOW ACTIN INITIATES THE MOTOR ACTIVITY OF MYOSIN. Virginie Ropars, Bin Zong, Paola Llinas, Tatiana Isabet, Lin Song, H. Lee Sweeney, Anne Houdusse 120-P lat 11:15 am STRUCTURAL BASIS FOR CALCIUM REGULATION OF THE MOTOR FUNCTION OF MYOSIN-5A. Mei Shen, Sanduo Zheng, Wen-Bo Zhang, Zekuan Lu, Xiang-dong Li 121-P lat 11:30 am STRUCTURAL DETERMINANTS OF MYOSIN I MECHANOSENSING: THE N TERMINAL REGION. Michael J. Greenberg , Tianming Lin, Henry Shuman, E. Michael Ostap 122-P lat 11:45 am MYOSIN 5 SIDE STEPS ALONG ACTIN. Jaime Ortega-Arroyo, Joanna Andrecka, Yasuharu Takagi, James R. Sellers, Philipp Kukura 123-P lat 12:00 pm PROBING LIPID VESICLE TRANSPORT IN 3D BY TEAMS OF MYOSIN VA MOTORS AT SUSPENDED ACTIN INTERSECTIONS IN VITRO. Andrew T. Lombardo , M. Yusuf Ali, Guy G. Kennedy, Kathleen M. Trybus, David M. Warshaw 124-P lat 12:15 pm MEASUREMENTS AND SIMULATIONS OF THE FISSION YEAST CYTOKINETIC RING TENSION DURING CONSTRICTION. Harvey F. Chin , Erdem Karatekin, Thomas D. Pollard, Ben O’Shaughnessy 125-P lat 12:30 pm A MINIMAL SYSTEM TO ESTABLISH MICROTUBULE-BASED CELL POLARITY IN VITRO . Núria Taberner , Pierre Recouvreux, Sophie Roth, Marileen Dogterom 10:45 am –12:45 pm , R oom 307/308 Platform RNA Structure, Translation, and Splicing Co-Chairs Jeffrey Kieft, University of Colorado, Denver Aaron Hoskins, University of Wisconsin-Madison 126-P lat 10:45 am BALANCED INTERACTIONS BETWEEN RIBOSOMAL SUBUNITS ALLOW RAPID LARGE-SCALE ROTATION. Lars V. Bock , Christian Blau, Andrea C. Vaiana, Helmut Grubmüller 127-P lat 11:00 am REAL-TIME OBSERVATION OF DNA RECOGNITION BY THE RNA-GUIDED ENDONUCLEASE CAS9 USING SINGLE- MOLECULE FRET. Digvijay Singh , Samuel H. Sternberg, Jingyi Fei, Jennifer A. Doudna, Taekjip Ha

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Biophysical Society 59 th Annual Meeting, Baltimore, Maryland