Biophysical Society 59th Annual Meeting Program Guide

4:00 pm –6:00 pm , B allroom I Symposium Emergent Properties and Collective Behaviors of Complex Systems

z-stacking in various biological systems such as cells, tissue, drosophila, C. elegans, bacteria and virus makes the Vutara 350 very versatile. Presenter Jeff Stuckey, Product Marketing Manager, Bruker Nano Surfaces 3:30 pm –5:00 pm , R oom 333 Early Careers Committee Meeting

Chair Aaron Dinner, University of Chicago 158-S ymp 4:00 pm SCALING LAWS GOVERNING GROWTH AND DIVISION OF SINGLE BACTERIAL CELLS. Aaron Dinner 159-S ymp 4:30 pm DRIVING WITH THE BRAKES ON: AN INCOHERENT TRANSCRIPTIONAL CIRCUIT PATTERNS THE DROSOPHILA EMBRYO. Angela DePace 160-S ymp 5:00 pm TEMPORAL FREQUENCY OF DIRECTIONAL SENSING AND COLLECTIVE MIGRATION IN DICTYOSTELIUM. Satoshi Sawai 161-S ymp 5:30 pm THE EMERGENCE OF HEART FAILURE AS A CONSEQUENCE OF MYOCARDIAL METABOLIC DYSFUNCTION. Daniel A. Beard 4:00 pm –6:00 pm , B allroom II Symposium Protein Evolution and Allosteric Networks Chair Corey Wilson, Yale University 162-S ymp 4:00 pm UNDERSTANDING ENZYME MOLECULAR EVOLUTION TOWARD THERMAL ADAPTATION USING MULTISTATE COMPUTATIONAL PROTEIN DESIGN. Corey J. Wilson 163-S ymp 4:30 pm ALLOSTERIC NETWORKS IN THROMBIN. Elizabeth Komives 164-S ymp 5:00 pm THE EVOLUTION OF ENZYME MECHANISMS AND FUNCTIONAL DIVERSITY. Janet Thornton N o A bstract 5:30 pm EXPERIMENTAL RECONSTRUCTION OF THE MECHANISMS OF ANCIENT PROTEIN EVOLUTION. Joe Thornton 4:00 pm –6:00 pm , B allroom III Symposium Cardiomyopathies and Contractile Proteins Chair Leslie Leinwand, University of Colorado 165-Symp 4:00 pm MYOSIN MYOPATHIES. Leslie Leinwand 166-Symp 4:30 pm POSTTRANSLATIONAL MODIFICATION OF TITIN DOMAINS AS A MAIN REGULATOR OF MYOCARDIAL STIFFNESS. Wolfgang A. Linke 167-Symp 5:00 pm MYBPC3 GENE THERAPY FOR NEONATAL SARCOMERIC CAR- DIOMYOPATHIES. Lucie Carrier

3:30 pm –5:00 pm , H all C, R oom A Exhibitor Presentation Wyatt Technology Corporation

The Light Scattering Toolkit for Biophysical Characterization: Lab Essentials for Enhancing Studies of Purification, Crystallization, Formulation, Conjugation, Conformation, and Interactions Biophysical techniques based on static and dynamic light scattering address many of the key analytical challenges associated with proteins, oligonucleotides, vesicles and other biomacromolecules. This workshop covers the following topics: 1. Batch DLS – traditional cuvette-based dynamic light scattering (DLS) is a fast, easy means of estimating macromolecular and nanoparticle size distributions to assess protein aggregation or the sizes of virus-like particles or drug delivery nanovehicles. In microwell-plate format, DLS is a high- productivity tool useful for optimizing formulation or crystallization conditions with minimal sample consumption or manual labor. 2. SEC-MALS and SEC-DLS – coupling of multi-angle static light scattering (MALS) and DLS detection to size-exclusion chromatography to assess molar mass, size, conformation and conjugation, in solution, independently of column calibration and non-ideal sample- column interactions. In addition to readily assessing aggregation and fragmentation in line with SEC purification, SEC-MALS analyzes protein conjugates such as glycoproteins or membrane proteins bound to surfactant micelles, determining protein oligomeric state and the mass of glycans, polysaccharides or surfactant modifying the protein. 3. FFF-MALS and FFF-DLS – coupling of MALS and DLS to a field- flow fractionation (FFF) device to achieve accurate characterization of macromolecules and nanoparticles from 1-1000 nm, even when soluble and insoluble components are both present in the solution. It does not employ a stationary phase; FFF separates without shear and with minimal surface interactions. FFF produces high-resolution size distributions thanks to true hydrodynamic separation upstream of the light scattering detectors. It also offers the benefits of post-separation downstream analysis by spectroscopy for additional information on samples. 4. CG-MALS – coupling MALS to a composition-gradient device results in a uniquely powerful system for characterizing complex biomolecular interactions, label-free and immobilization-free. Because MALS measures molar masses it is one of the most useful techniques for analyzing multi-domain, multi-protein interactions that go beyond standard 1:1 interactions including systems exhibiting cooperativity and allostery. CG- MALS determines the affinity and absolute molecular stoichiometry of self and/or heteroassociating systems from pM to mM. Presenter Stephanie Cope, Applications Scientist, Wyatt Technology Corporation 4:00 pm –5:00 pm , R oom 301/302/303 Career Center Workshop Beyond the Bench: Preparing for Your Career Transition in the Life Sciences There are numerous alternative career options for the seasoned bench scientist who may have decided to take his/her talents and apply them in a new direction. This transition can be accomplished without having to matriculate in another graduate program, and this session explores the how’s and why’s of making such a transition. Be prepared to talk about the role you are thinking about moving into, why you may have chosen this alternative path, and what successes you may have had thus far.

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Biophysical Society 59 th Annual Meeting, Baltimore, Maryland