Biophysical Society 60th Annual Meeting

1702-Plat 8:45 am CRYOEM-GUIDED ITERATIVE MOLECULAR DYNAMICS - ROSETTA PROTEIN STRUCTURE REFINEMENT PROTOCOL IMPROVES PROTEIN MODEL QUAL- ITY.  Steffen Lindert , Melanie Marlett 1703-Plat 9:00 am STRUCTURE-BASED PREDICTION OF PROTEASE MULTISPECIFICITY USING COMPUTATIONAL PROTEIN DESIGN.  Sagar D. Khare , Aliza Rubenstein, Manasi Pethe 1704-Plat 9:15 am PROTEIN SEQUENCE OPTIMIZATION WITH A POLARIZABLE FORCE FIELD: INSIGHTS FROM PDZ DOMAINS.  Jacob M. Litman, Young Joo Sun, Titus Hou, Stephen D. LuCore, Nicolas Panel, Thomas Simonson, Ernesto J. Fuentes, Michael J. Schnieders 1705-Plat 9:30 am DE NOVO DESIGN AND BIOPHYSICAL CHARACTERIZATION OF AN AFFIN- ITY-ENHANCED PROTEIN DISPLAYING THE STRUCTURE OF THE BROADLY NEUTRALIZING HIV-1 2F5 ANTIBODY EPITOPE.  Isabelle Freire Tabosa Viana , Eduardo Nascimento, Jodi Craigo, Marco Krieger, Robbie Mailliard, Rafael Dhalia, Roberto Lins, Ernesto TA Marques 1706-Plat 9:45 am A TOOL TO INTEGRATE USER EXPERTISE INTO BUILDING ATOMIC LEVEL MODELS FOR LARGE BIOMOLECULAR SYSTEMS.  Till Rudack , Ryan McGreevy, Marc Siggel, Klaus Schulten 1707-Plat 10:00 am RESIDUE ENVIRONMENT SCORE FOR SELECTING PROTEIN STRUCTURE MODELS AND PROTEIN-PROTEIN DOCKING MODELS.  HyungRae Kim Platform Protein Assemblies 8:15 am - 10:15 am, Room 515A Co-Chairs James Cole, University of Connecticut Alasdair Steven, NIH 1708-Plat 8:15 am SPECIFIC INTERACTION OF A NATURALLY OCCURRING AMYLOIDOGENIC FRAGMENT OF STREPTOCOCCUS MUTANS ADHESIN P1 WITH INTACT P1 ON THE CELL SURFACE MEASURED BY SOLID STATE NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY.  Joanna Long , Wenxing Tang, Paula Crowley, L. Jeannine Brady 1709-Plat 8:30 am REGULATION OF PKR BY RNA: FORMATION OF ACTIVE AND INACTIVE DIMERS.  Bushra Husain, Stephen Hesler, James Cole 1710-Plat 8:45 am EDUCATION TRAVEL AWARDEE GOVERNING PRINCIPLES OF MULTIPROTEIN COMPLEX FORMATION ON THE CELL MEMBRANES: AN INVESTIGATION USING SINGLE-MOLECULE RESOLUTION SPATIO-TEMPORAL STOCHASTIC COMPUTER SIMULATIONS AND ANALYTICAL CALCULATIONS.  Osman N. Yogurtcu , Margaret E. Johnson 1711-Plat 9:00 am CHARACTERIZATION OF NOVEL SPLIT-FLUORESCENT PROTEINS AND QUANTITATIVE ANALYSIS OF THEIR SELF-ASSEMBLY PROCESS.  Tugba Koker , Anthony Fernandez, Arunima Kolekar, Fabien Pinaud 1712-Plat 9:15 am A NEW DIMENSION OF DETECTION IN ANALYTICAL ULTRACENTRIFUGA- TION WITH FLUORESCENCE DETECTION USING PHOTOSWITCHABLE FPS AS TIME DOMAIN PROBES.  Huaying Zhao , George Patterson, Peter Schuck

1713-Plat 9:30 am MOLECULAR ARCHITECTURE OF THE NUP82 COMPLEX, THE CYTOPLAS- MIC MRNA EXPORT PLATFORM IN THE NUCLEAR PORE COMPLEX.  Seung Joong Kim , Javier Fernandez-Martinez, Yi Shi, Paula Upla, Riccardo Pellarin, Ilan E. Chemmama, Ilona Nudelman, David L. Stokes, Brian T. Chait, Andrej Sali, Michael P. Rout 1714-Plat 9:45 am LOCAL GEOMETRY AND EVOLUTIONARY CONSERVATION OF PROTEIN SURFACES REVEAL THE MULTIPLE RECOGNITION PATCHES IN PROTEIN- PROTEIN INTERACTIONS.  Elodie Laine , Hugues Ripoche, Alessandra Carbone 1715-Plat 10:00 am RETINOSCHISIN AT 4 Å RESOLUTION FROM CRYO-EM: A JUNCTIONAL MODEL OF BACK-TO-BACK OCTAMERS FOR ADHESION IN THE RETINA.  Gökhan Tolun, Camasamudram Vijayasarathy, Rick Huang, Yong Zeng, Yan Li, Paul A. Sieving, J. Bernard Heymann, Alasdair C. Steven Platform Mechanosensation 8:15 am - 10:15 am, Room 515B Co-Chairs Dolores Bozovic, University of California, Los Angeles Hongxia Fu, Harvard Medical School 1716-Plat 8:15 am SINGLE-MOLECULE ACTIVATION BY FLOW: FORCE-INDUCED CONFORMA- TIONAL TRANSITIONS REGULATE THE LIGAND BINDING AFFINITY OF A LARGE, POLYMERIC MECHANOSENSOR PROTEIN.  Hongxia Fu , Yan Jiang, Darren Yang, Wesley P. Wong, Timothy A. Springer 1717-Plat 8:30 am BILAYER-MEDIATED STRUCTURAL TRANSITIONS IN THE TREK-2 MECHA- NOSENSITIVE K2P CHANNEL.  Prafulla Aryal , Viwan Jarerattanachat, Stephen J. Tucker, Mark S.P. Sansom 1718-Plat 8:45 am MAGNETIC NANOPARTICLE STIMULATION FOR DETECTION OF HIGH-OR- DER MODE-LOCKING IN HAIR CELLS OF THE INNER EAR.  Dolores Bozovic , Michael Levy 1719-Plat 9:00 am STRETCH-GATED ION CHANNELS IN NEURONAL MECHANORECEPTORS.  Slav N. Bagriantsev , Eve R. Schneider, Evan O. Anderson, Jon Matson, Elena O. Gracheva 1720-Plat 9:15 am BIOPHYSICAL FACTORS THAT PROMOTE MECHANICALLY-INDUCED AC- TION POTENTIALS IN NEOCORTICAL AND HIPPOCAMPAL PYRAMIDAL NEURONS.  Yury A. Nikolaev, Peter J. Dosen, Derek R. Laver, Dirk F. Van Helden, Owen P. Hamill 1721-Plat 9:30 am CPOW TRAVEL AWARDEE INFLAMMATORY CYTOKINE IL-1α UP-REGULATES PIEZO1 AND HYPER- SENSITIZES CHONDROCYTES TO COMPRESSION. Whasil Lee , Holly Leddy, Amy McNulty, Farshid Guilak, Wolfgang Liedtke 1722-Plat 9:45 am STRUCTURAL AND FUNCTIONAL CHARACTERIZATIONS OF THE MECHA- NOSENSITIVE PIEZO CHANNEL.  Bailong Xiao 1723-Plat 10:00 am BENDING PIEZO1: THE EFFECT OF AMPHIPATHS ON THE GATING OF A MECHANOSENSITIVE CHANNEL.  Charles D. Cox , Boris Martinac

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