Biophysical Society 60th Annual Meeting

Platform Force Spectroscopy and Scanning Probe Microscopy 8:15 am - 10:15 am, Room 515B Co-Chairs Pieter De Beule, International Iberian Nanotechnology Laboratory, Portugal A. Catalina Velez-Ortega, University of Kentucky 2545-Plat 8:15 am ACOUSTIC FORCE SPECTROSCOPY: AN INSTRUMENT TO PERFORM HIGHLY PARALLEL SINGLE MOLECULE MEASUREMENTS.  Gerrit Sitters , Felix Oswald, Douwe Kamsma, Jerom Langeveld, Willem Peutz, Erwin Peterman, Gijs Wuite, Olivier Heyning 2546-Plat 8:30 am A NOVEL METHOD FOR MULTIPLEXED NANOMETRIC BEAD TRACKING.  Thomas Brouwer , John van Noort 2547-Plat 8:45 am SIMULTANEOUS ADVANCED MICROSCOPIES FOR LIVE CELL SIGNALING DYNAMICS INVESTIGATIONS.  Adelaide Miranda, Marco Martins, Pieter A. A. De Beule 2548-Plat 9:00 am VIDEO-BASED FORCE DETECTION IN OPTICAL TWEEZERS TO MEASURE DNA TRANSLOCATION THROUGH SI-NX AND LIPID-COATED NANOPORES.  Andy Sischka , Sebastian Knust, Lukas Galla, Andreas J. Meyer, Andre Spiering, Michael Mayer, Adam R. Hall, Peter Reimann, Karsten Gall, Dario 9:15 am ADAPTIVE HOPPING PROBE ION CONDUCTANCE MICROSCOPY OF LIVE CELLS AT ~5-10 NM RESOLUTION.  A. Catalina Velez-Ortega , Oleg Belov, Pavel Novak, Samir A. Rawashdeh, Yuri E. Korchev, Gregory I. Frolenkov 2550-Plat 9:30 am DIRECT OBSERVATION OF TRANSITION PATHS DURING THE FOLDING OF PROTEINS AND NUCLEIC ACIDS.  Krishna Neupane, Daniel AN Foster, Derek R. Dee, Hao Yu, Feng Wang, Michael T. Woodside 2551-Plat 9:45 am EQUILIBRIUM FOLDING OF AN INDIVIDUAL BACTERIORHODOPSIN INTO AND OUT OF ITS NATIVE LIPID BILAYER RESOLVES ENERGY LANDSCAPES AND HIDDEN DYNAMICS.  Hao Yu , Matthew G. W. Siewny, Devin T. Edwards, Aric W. Sanders, Thomas T. Perkins 2552-Plat 10:00 am BINDING MECHANISM OF PURINE NUCLEOTIDES TO MITOCHONDRIAL UNCOUPLING PROTEINS EXPLORED BY RECOGNITION FORCE SPECTROS- COPY. Melanie Koehler , Gabriel Macher, Anne Rupprecht, Rong Zhu, Hermann J. Gruber, Elena E. Pohl, Peter Hinterdorfer Platform Biosensors 8:15 am - 10:15 am, Room 501ABC Co-Chairs Loredana Casalis, Elettra Sincrotrone Trieste, Italy Sergey Sekatskii, École Polytechnique Fédérale de Lausanne, Switzerland 2553-Plat 8:15 am INTERNATIONAL TRAVEL AWARDEE CONTROLLING THE NANOSCOPIC CONFINEMENT OF ENZYMES INSIDE CLYA NANOPORES FOR SINGLE-PROTEIN STUDIES.  Annemie Biesemans , Misha Soskine, Giovanni Maglia Anselmetti 2549-Plat

2534-Plat 9:30 am MAPPING MOTIONS AND STRUCTURE TO A STATE NECESSARY FOR OLIGOMERIZATION OF A LARGE GTPASE: A JOINT SAXS, NSE, EPR AND FRET STUDY.  Thomas-Otavio Peulen , Carola S. Hengstenberg, Ralf Biehl, Mykola Dimura, Alessandro Valeri, Semra Ince, Tobias Vöpel, Bela Farago, Holger Gohlke, Christian Herrmann, Johann Klare, Andreas Stadler, Claus A.M. Seidel 2535-Plat 9:45 am TONB BINDING PRODUCES ALLOSTERIC CHANGES IN THE OUTER LOOPS AND SUBSTRATE BINDING SITE OF THE TBDT BTUB.  Arthur K. Sikora , David Cafiso 2536-Plat 10:00 am CONFORMATIONAL DYNAMICS AND STRUCTURE UNDERLIE THE NOVEL LIGAND BINDING SPECIFICITY OF A TIAM1 PDZ DOMAIN MUTANT.  Ernesto J. Fuentes , Xu Liu, David C. Speckhard, Tyson R. Shepherd, Sarah R. Hengel, Liping Liping, C. Andrew Fowler, Lokesh Gakhar Platform Protein-Nucleic Acid Interaction 8:15 am - 10:15 am, Room 515A Co-Chairs Timothy Craggs, University of Oxford, United Kingdom Steve Harvey, University of Pennsylvania 2537-Plat 8:15 am HOW STRUCTURE-SPECIFIC DNA-BINDING PROTEINS RECOGNISE THEIR SUBSTRATES.  Timothy D. Craggs , Marko Sustarsic, Majid Mosayebi, Hendrik Kaju, Johannes Hohlbein, Phillip C. Biggin, Jonathan P.K. Doye, Achilles N. Kapanidis 2538-Plat 8:30 am FLIPPING BY DNA BOUND PROTEINS OCCURS THROUGH RAPID REBIND- ING. Mahipal Ganji , Margreet Docter, Stuart F.J. Le Grice, Elio Abbondanzieri 2539-Plat 8:45 am STEPWISE NUCLEOSOME TRANSLOCATION BY RSC REMODELING COM- PLEXES.  Bryan T. Harada , William L. Hwang, Sebastian Deindl, Blaine Bartholomew, Xiaowei Zhuang 2540-Plat 9:00 am PROBING HELICASE DYNAMICS ON NUCLEIC ACIDS THROUGH FLUORES- CENCE-FORCE MEASUREMENTS.  Chang-Ting Lin , Meigang Gu, Charles M. Rice, Virginia A. Zakian, Taekjip Ha Ha 2541-Plat 9:15 am THE SCRUNCHWORM HYPOTHESIS: TRANSITIONS BETWEEN A-DNA AND B-DNA PROVIDE THE DRIVING FORCE FOR GENOME PACKAGING IN DOUBLE-STRANDED DNA BACTERIOPHAGES.  Stephen C. Harvey , James T. Waters, James C. Gumbart, Harold D. Kim 2542-Plat 9:30 am TFAM REGULATES MITOCHONDRIAL TRANSCRIPTION THROUGH SEQUENCE-SPECIFIC DNA LOOPING.  Divakaran Murugesapillai , Maria F. Lodeiro, Louis J. Maher III, Craig E. Cameron, Mark C. Williams 2543-Plat 9:45 am VISUALIZING THE ASSEMBLY OF DNA CONDENSATION CLUSTERS BY SMC USING SINGLE-MOLECULE MICROSCOPY.  Hyeongjun Kim , Joseph J. Loparo 2544-Plat 10:00 am SINGLE MOLECULE IMAGING OF P53’S DYNAMIC INTERACTION WITH CHROMATIN.  Vincent Wong, Zhe Liu, Sam Peng, Charles Kenworthy, Wei-Li Liu, Robert A Coleman

W E

D N

E S

D A Y

135