Biophysical Society 60th Annual Meeting

the velocity of the deformation- and this mechanical component can sometimes be lost or ignored in certain experimental setups and tech- niques. In fact, this viscous response may prove just as enlightening to cell mechanics as the elastic response more commonly measured alone until recently. This talk will discuss these important issues that must be considered when AFM techniques are applied to cells and other biologi- cal materials. Speaker Sophia Hohlbauch, Asylum Research, an Oxford Instruments Company 1:30 pm–3:00 pm KinTek Corporation Why You Should Fit Kinetic and Equilibrium Binding Data Using Kintek Explorer Software KinTek Explorer software offers the fastest, most dynamic and robust method of fitting kinetic or equilibrium binding data. Based on fast numerical integration of rate equations, data are fit without the often- inaccurate approximations needed to derive equations. Rather than fit- ting data to extract “observed rates” or Eigenvalues, which must be then interpreted in second step, KinTek Explorer yield rate and equilibrium constants directly while accounting for both the rate and amplitude of observable reactions. By modeling the experiments exactly as per- formed, all details of the experimental setup are included, eliminating errors in interpretation. Moreover, multiple experiments can be fit simultaneously to a single unifying model. Fast dynamic simulation using proprietary methods for numerical integration allows you to explore parameter space and learn kinetics. Don’t be fooled by other vendors pretending to do the same. Only KinTek Explorer offers such robust and dynamic data fitting. In this presentation, Professor Johnson will introduce the theory and operation of the software to show you how easy it is to fit data to any model you care to input. Examples of experiments that can be fit include: transient and single turnover stopped-flow kinetics, steady state kinetics, slow onset inhibition, equilibrium titrations, rapid- quench-flow kinetics, temperature dependence, voltage-dependent rate constants. In addition time-resolved absorbance or fluorescence and pH-dependent spectra can be analyzed by singular value decom- position to yield spectra and time- or pH-dependence of each spe- cies. In addition to describing KinTek Explorer’s basic features, Dr. Johnson will introduce new features and will be available to help you to fit your own data. Learn about what you are missing in your own data fitting. See www.kintek-corp.com for more information. Speaker Kenneth A Johnson, President, KinTek Corporation; Professor of Biochemistry, University of Texas at Austin

3:30 pm–5:00 pm Bruker Nano Surfaces Advances in Live Super-resolution Imaging Using the Vutara 352 Microscope Super-resolution microscopy has made a significant impact in the field of biological imaging by enabling a ten-fold improvement in spatial resolu- tion over traditional light microscopy techniques. Most of the imaging has been so far targeted at fixed specimens with a few live cell applica- tions. The Vutara 352 microscope has been engineered towards live-cell imaging by enhancing spatial and temporal resolution in single molecule localization super-resolution. The sCMOS detector in the Vutara 352 enables imaging at 800 fps at full ROI and at video frame rates at reduced ROI. Two color simultaneous imaging can be applied in both super-res- olution live cell and 3D particle tracking experiments. The biplane based detection path enables imaging thicker samples such as whole mount Drosophila and offers deeper penetration into tissues. The Vutara 352 also includes real time localization along with several statistical and live cell analysis features for processing data. In summary, the Vutara 352 microscope is a powerful super-resolution imaging and analysis tool. Speaker Manasa Gudheti, Applications Scientist at Bruker – Fluorescence Microscopy Business Patch clamp electrophysiology has matured from a highly specialized scientific technique to a recognized method used to address a variety of experimental questions. Sutter Instrument introduces a highly flex- ible, intuitive patch clamp instrumentation and software package that enables the experimenter to quickly set up and perform routine tasks, yet remains highly configurable to meet the demands of the experienced electrophysiologist. We will demonstrate how the IPA™ Integrated Patch Amplifier and SutterPatch™ software can be used for a variety of commonly performed assays, including the characterization of an ionic current and the record- ing of synaptic events in tissue slices. We will also highlight how the IPA and SutterPatch software provide easy access and flexibility to perform and fine-tune the most challenging acquisition and analysis scenarios. Building on the basic pipette pulling tutorials presented at the 2015 user meeting and a mid-year webinar, we will further teach advanced tech- niques that enable the user to create specialized pipette morphologies for unique applications. There will be plenty of opportunity for discussion with hosts and speak- ers from the Sutter Instrument Tech Support Team. Who should attend? • Electrophysiologists who use amplifiers, micropipettes and micro- manipulators for patch clamp, sharp electrode or extracellular recordings. • Researchers who perform microinjections, including nuclear trans- fer, sperm injection and application of substances into cell cultures or intact organisms. Speakers Jan Dolzer, Tech Support and Product Development, Sutter Instrument Gregory Hjelmstad, Tech Support and Product Development, Sutter Instrument 5:30 pm–7:00 pm Sutter Instrument Scientists Empowering Scientists

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