Biophysical Society 60th Annual Meeting

New Member Welcome Coffee 10:15 am - 11:15 am, Room 404AB

926-Symp12:15 pm DETYROSINATED MICROTUBULES BEAR LOAD AND TRANSMIT MECHANI- CAL FORCE IN CARDIOMYOCYTES.  Patrick Robison, Matthew Caporizzo, Alexey Bogush, Kenneth Margulies, Benjamin Prosser Symposium Future of Biophysics 10:45 am - 12:45 pm, Petree Hall D Co-Chairs Vasanthi Jayaraman, University of Texas Healtah Science Center E. Michael Ostap, University of Pennsylvania No Abstract 10:45 am ENGINEERING BIOMIMETIC MATRICES TO UNDERSTAND BRAIN/TUMOR INTERACTIONS DURING METASTASIS.  Kimberly Stroka No Abstract 11:15 am COMBINATORIAL REGULATION OF BAR DOMAIN PROTEINS AT THE INTER- FACE BETWEEN THE CYTOSKELETON AND MEMBRANES.  David Kast No Abstract 11:45 am NASCENT AND ANTIMICROBIAL PEPTIDES THAT TARGET THE RIBOSOMAL EXIT TUNNEL TO BLOCK PROTEIN SYNTHESIS IN BACTERIA.  Axel Innis No Abstract 12:15 pm MECHANISM OF GATING AND ION SELECTIVITY IN ASIC/ENAC/DEG CHANNELS.  Isabelle Baconguis Platform Voltage-gated K Channels and Mechanisms of Voltage Sensing and Gating I 10:45 am - 12:45 pm, Room 502A Co-Chairs Peter Larsson, University of Miami Gail Robertson, University of Wisconsin-Madison 927-Plat 10:45 am N-ARACHIDONOYL TAURINE RESCUES DIVERSE LONG QT SYNDROME- ASSOCIATED MUTATIONS IN THE CARDIAC IKS CHANNEL.  Sara I. Liin , Johan E. Larsson, Rene Barro-Soria, Mark A. Skarsfeldt, Bo H. Bentzen, H Peter Larsson 928-Plat 11:00 am THE F-ACTIN BINDING PROTEIN TRIOBP-1 REGULATES HERG K + CHAN- NELS.  Ashley A. Johnson , Dave Jones, Elon C. Roti Roti, Gail Robertson, Matthew Trudeau 929-Plat 11:15 am A NOVEL SITE OF COMPETITIVE PIP2 AND CALMODULIN INTERACTION TO KCNQ1 C-TERMINUS HELIX B IS CRUCIAL FOR IKS CHANNEL ACTIVITY.  William S. Tobelaim , Meidan Dvir, Guy Lebel, Meng Cui, Tal Buki, Asher Peretz, Diomedes Logothetis, Joel Hirsch, Bernard Attali 930-Plat 11:30 am MOLECULAR DYNAMICS SIMULATIONS OF KIR2.2-CHOLESTEROL INTER- ACTIONS.  Nicolas Barbera , Manuela A. Ayee, Belinda S. Akpa, Irena Levitan 931-Plat 11:45 am CRYO-EM STRUCTURE OF THE BK ION CHANNEL IN A LIPID MEMBRANE.  Liguo Wang , Lige Tonggu, Xi Zhan

All new and prospective Biophysical Society members are invited to par- ticipate in an informal gathering to meet members of the Society’s coun- cil and committees, find out about the Society’s activities, get acquainted with other new members, and enjoy refreshments. Current members are encouraged to come meet the new members. Exhibitor Presentation Wyatt Technology Corporation 10:30 am - 12:00 pm, Room 513 Get it Right the First Time - Enhancing Protein Binding and Structural Studies with the Light-Scattering Toolkit Biophysical binding studies utilizing surface plasmon resonance (SPR), biolayer interferometry (BLI), isothermal titration calorimetry (ITC), and related techniques are central to the study of protein-protein, protein- DNA and similar biomolecular interactions. Though these are well-estab- lished techniques, in a variety of circumstances, binding measurements may be ambiguous or even fail to provide useful data. Wasted measure- ments can end up being costly in terms of consumables and time. Small-angle X-ray scattering (SAXS) and small-angle neutron scattering (SANS) are powerful techniques for studying biomolecular structure. SAXS and SANS usually require precious beam time at large facilities, leav- ing little room for error where the sample preparation is concerned. Poor samples provide poor SAXS/SANS data, but the opportunity to utilize the X-ray or neutron beammay never be recovered. One thing that SAXS and SANS have in common with SPR, BLI and ITC, is the urgent need to verify sample quality and aggregation state in solution prior to carrying out structural or binding measurements. This seminar discusses a suite of complementary techniques, all based on light scat- tering, that are useful in assessing and troubleshooting many of the un- derlying characterization issues. Multi-angle light scattering (MALS) and dynamic light scattering (DLS) can help researchers assess solution quality prior to running binding or structural experiments, qualify aggregation behavior of analytes, and characterize complex interactions that may not be amenable to standard characterization methodology. Judicious use of the biophysical light-scattering toolkit is essential for robust and reliable interaction and structure studies. Speaker Sophia Kenrick, Application Scientist, Wyatt Technology Corporation Symposium Mechanosending and Mechanosignaling in Muscle 10:45 am - 12:45 pm, Petree Hall C Chair Olga Mayans, University of Konstanz, Germany 923-Symp10:45 am MECHANOSENSITIVE STRUCTURAL STATES OF TITIN.  Zsolt Mártonfalvi, Pasquale Bianco, Katalin Naftz, Dorina Kőszegi, György Ferenczy, Miklós S. Kellermayer 924-Symp11:15 am TITIN(S): TOWARDS AN ATOMIC UNDERSTANDING OF MECHANOSEN- SORY EVENTS IN THE ELASTIC SCAFFOLDS OF THE MUSCLE SARCOMERE.  Olga Mayans , Jennifer Fleming, Rhys Williams, Barbara Franke, Hang Lu, Guy Berrian 925-Symp11:45 am MECHANO-CHEMO-TRANSDUCTION IN CARDIOMYOCYTES DURING BEAT- TO BEAT CONTRACTION UNDER MECHANICAL LOAD.  Ye Chen-Izu

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