Biophysical Society 61st Annual Meeting | Program Guide

823-Plat 8:30 am STABILIZATION OF VARIABLE DOMAIN DIMERS OF AN IMMUNOGLOBU- LIN LIGHT CHAIN INHIBITS FORMATION OF AMYLOID FIBRILS.  Shannon R. Esswein , Boris Brumshtein, David S. Eisenberg 824-Plat 8:45 am AN INTERNAL DISULFIDE LOCKS A MISFOLDED AGGREGATION-PRONE INTERMEDIATE IN CATARACT-LINKED MUTANTS OF HUMAN GAMMA-D CRYSTALLIN.  Eugene Serebryany , Jaie C. Woodard, Bharat V. Adkar, Mo- hammed Shabab, Jonathan A. King, Eugene I. Shakhnovich 825-Plat 9:00 am BIOPHYSICAL AND EVOLUTIONARY ASPECTS OF DOMAIN SWAPPING IN THE FORKHEAD DOMAIN OF HUMAN FOXP PROTEINS.  Exequiel Medina, Javiera Reyes, Pablo Villalobos, Elizabeth A. Komives, Jorge Babul, Cesar A. Ramirez-Sarmiento 826-Plat 9:15 am UNDERSTANDING PROTEIN DOMAIN-SWAPPING IN THE CYSTATIN- MONELLIN FAMILY OF PROTEINS.  Nahren Manuel Mascarenhas, Shachi Gosavi 827-Plat 9:30 am USING HIGH PRESSURE NMR TO STUDY FOLDING COOPERATIVITY AND KINETICS OF PROTEIN L9.  Yi Zhang , Soichiro Kitazawa, Ivan Peran, Natalie Stenzoski, Scott McCallum, Daniel Raleigh, Catherine Royer 828-Plat 9:45 am INTRACELLULAR MODULATION OF PROTEIN FOLDING STABILITY PROBED BY A NOVEL FOLDING REPORTER.  David Gnutt , Jonas Ahlers, Benedikt König, Simon Ebbinghaus 829-Plat 10:00 am MODULATING SOD1 FOLDING LANDSCAPES WITH TARGETED MOLECU- LAR BINDERS.  David N. Bunck , Beatriz Atsavapranee, Katrine Museth, James Heath Exhibitor Presentation TA Instruments 8:30 am – 10:00 am, Room 221 Instrumentation and Experimental Design for Utilization of ITC and IMC Techniques for Characterization of Biopharmaceuticals Eight out of the top ten drugs worldwide in 2016 are biopharmaceuti- cals. Microcalorimetry is a powerful tool in the characterization of their structure and stability. Isothermal titration calorimetry (ITC) has long been the gold standard technique for exploring the binding of any drug to its target molecule. Recent publications describing the advantages of enthalpy screening utilizing the advances in ITC hardware and software exemplified by the Affinity ITC instruments from TA Instruments have shown that microcalorimetry remains a powerful tool in characterizing biopharmaceuticals. Isothermal Calorimetry (IMC) has the sensitivity and flexibility to fully characterize formulation stability and aggregation kinet- ics of even the high concentration (200-300 mg/mL) biopharmaceutical formulations. The sensitivity and flexibility of the TAM IV microcalorim- etry system has shown the ability to rapidly characterize even the slowest degradation or aggregation reactions taking place in these biopharma- ceutical formulations without diluting the sample. Attendees will partici- pate in a wide ranging discussion of these powerful techniques including a full detailed description of the new Affinity ITC and TAM IV hardware along with a Q&A session focused on experimental design considerations for successfully performing enthalpy screening and biopharmaceutical formulation stability testing. Join your colleagues in this in-depth discus- sion of how ITC and IMC can speed up the candidate selection process and benefit overall lab productivity in any drug discovery or development effort. Speaker Dile Holton, Microcalorimetry Product Manager, TA Instruments

813-Plat 9:45 am RECOVERY OF CALCIUM ACTIVITY AND CONTRACTION IN MODELS OF DILATED CARDIOMYOPATHY.  Sonette Steczina , Soley Olafsson, Galina Flint, Jil Tardiff, Michael Regnier, Farid Moussavi-Harami 814-Plat 10:00 am ACUTE REDUCTION OF DESMIN INTERMEDIATE FILAMENTS ALTERS MYOCYTE MECHANICS AND EXCITATION-CONTRACTION COUPLING.  Julie Heffler , Matthew A. Capporizzo, Alexey I. Bogush, Patrick Robison, Benjamin L. Prosser Platform Force Spectroscopy 8:15 am - 10:15 am, Room 206/207 DIRECT OBSERVATION OF TRANSITION-STATE DYNAMICS DURING FOLD- ING REACTIONS.  Noel Q. Hoffer , Krishna Neupane, Michael T. Woodside 816-Plat 8:30 am MECHANICAL CHARACTERIZATION OF THE HIV-1 RNA HAIRPIN USING AN ATOMIC FORCE MICROSCOPE.  Robert Walder , William J. Van Patten, Ty W. Miller, Thomas T. Perkins 817-Plat 8:45 am UNCOVERING THE FORCES BETWEEN NUCLEOSOMES USING A DNA ORIGAMI FORCE SPECTROMETER.  Jonas J. Funke , Philip Ketterer, Corinna Lieleg, Sarah Schunter, Philipp Korber, Hendrik Dietz 818-Plat 9:00 am EXPLORING NUCLEIC ACID MECHANICS USING MULTIPLEXED MECHA- NOCHEMISTRY ASSAY (MMA).  Bhavik Nathwani , Darren Yang, Wesley Wong, WilliamM. Shih 819-Plat 9:15 am E-CADHERIN FUNCTIONS AS A DESMOGLEIN TRANSPORTER THAT FACILI- TATES ASSEMBLY OF NASCENT DESMOSOMES.  Omer M. Shafraz , Sara N. Stahley, Kannan Sankar, Robert L. Jernigan, Andrew P. Kowalczyk, Sanjeevi Sivasankar 820-Plat 9:30 am FORCES AND DYNAMICS IN PROTEIN TRANSLOCATION THROUGH THE BACTERIAL TRANSLOCON.  Anny Fis , Andreas Karner, Roland Kuttner, Johannes Preiner, Mirjam Zimmermann, Hermann J. Gruber, Peter Pohl, Peter Hinterdorfer 821-Plat 9:45 am A MULTI-TOOL MOUSE MODEL TO STUDY THE ELASTICITY OF NATIVE TITIN.  Jaime Andres Rivas-Pardo , Zsolt Mártonfalvi, Aitor Manteca, Ed- ward C. Eckels, Daniel J. Echelman, Miklós S.Z. Kellermayer, Jorge Alegre- Cebollada, Wolfgang Linke, Julio M. Fernandez Platform Protein Stability, Folding, and Chaperones II 8:15 am - 10:15 am, Room 208/209 Co-Chairs Cesar Ramirez, Universidad de Chile Shachi Gosavi, National Centre for Biological Sciences-TIFR, India 822-Plat 8:15 am FOLDING IN PIECES. Weiwei Kuo, Upneet Kaur, Daniel Deredge, Cameron J. Kilcoyne, Eugenia M. Clerico, Patrick L. Wintrode, Lila M. Gierasch, Anne Gershenson Co-Chairs Peter Hinterdorfer, Johannes Kepler University Linz, Austria Bhavik Nathwani, Harvard University 815-Plat 8:15 am

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