Biophysical Society 62nd Annual Meeting | Program Guide

Exhibitor Presentation Cellular Dynamics International, a FUJIFILM company 10:30 am–12:00 pm, Exhibit Hall, Room 6 Using Human iPSC-Derived Cell-Types in Novel Functional Assays, Disease Modeling, and Drug Discovery The availability of donor-specific induced pluripotent stem (iPS) cells, coupled with gene-editing techniques, is enabling new insights into the molecular basis and mechanisms of human disease. Join us as we describe how Cellular Dynamics’ cryopreserved iPSC-derived cell-types have been used to develop disease models with innate or introduced mutations. 10:30 am: Dr. Leonard Kaczmarek from Yale University will begin the talks by describing the use of stem cells in understanding mechanisms of ataxias and epilepsy, highlighting human iPSC-derived neurons harboring mutations in the KCNT1 Slack channel. 11:00 am: Dr. Kile Mangan from Cellular Dynamics International will follow with a talk on utilizing novel functional assays with high-definition multielectrode arrays (HD-MEAs: MaxWell Biosystems) to uncover phe- notypic differences in neurons harboring single-nucleotide disease muta- tions (alpha synuclein A53T Parkinson’s Disease) or in normal control following pharmacological perturbation.   11:30 am: ​Recent advances in cardiac tissue engineering have increased significantly cell functional across electrophysiological, Ca2+ handling, and contractility. The third presentation of this session will discuss these advances and provide exemplar laboratory case studies highlighting the increased functionality and experimental implementation.   Speakers Leonard Kaczmarek, Professor of Pharmacology and Cellular and Molecular Physiology, Yale University Kile Mangan, Group Leader, Application Development, Cellular Dynamics International, a FUJIFILM company TBD, Cardiomyocyte Bioengineering Applications Specialist International Relations Committee Meeting 10:30 am–12:30 pm, South, Level Three, Room 312 Symposium Translational Biophysics 10:45 am–12:45 pm, North, Lower Lobby, Room 24 Co-Chairs Melanie Cocco, University of California, Irvine Shankar Subramaniam, University of California, San Diego 120-Symp 10:45 am AGENTS TO BLOCK THE NEURITE OUTGROWTH INHIBITOR (NOGO, RTN4) INSPIRED BY THE STRUCTURE. Melanie J. Cocco 121-Symp 11:15 am BIOPHYSICS-ENABLED TRANSLATIONAL MEDICINE.  Donald Ingber 122-Symp 11:45 am ROLE OF MATRIX PROTEINS IN BALANCING TISSUE STIFFNESS AND INFLAMMATION IN FIBROSIS.  Shyni Varghese 123-Symp 12:15 pm MECHANISMS DEFINING THE NEURONAL STATE SPACE.  Shankar Subramaniam , Andrew Caldwell, Vipul Bhargava, Dinorah Friedman-Morvinski, Qing Lu, Shauna Yuan, Douglas Galasko, Inder Verma, Steven Wagner

Symposium Protein and RNA Phase Separation 10:45 am–12:45 pm, North, Lower Lobby, Room 25 Co-Chairs Simon Alberti, Max Planck Institute, Germany Tanja Mittag, St. Jude Children’s Research Hospital 124-Symp 10:45 am ORGANIZING LIVING MATTER: THE ROLE OF PHASE TRANSITIONS IN CELL BIOLOGY AND DISEASE.  Simon Alberti 125-Symp 11:15 am DYSREGULATION OF PHASE SEPARATION IN CANCER.  Tanja Mittag 126-Symp 11:45 am LIGHTING UP INTRACELLULAR PHASE SPACE.  Clifford P. Brangwynne 127-Symp 12:15 pm PHYSICAL MECHANISMS OF CELL ORGANIZATION ON MICRON LENGTH SCALES. Michael K. Rosen Platform Ligand-gated Channels 10:45 am–12:45 pm, South, Level Two, Room 207/208 Co-Chairs Ljudmila Katchan, Leibniz-Forschungsinstitut for Molecular Pharmacology, Germany Nadine Mundt, University of California, Berkeley 128-Plat 10:45 am ION SELECTIVITY IN ACID-SENSING ION CHANNELS AND EPITHELIAL SO- DIUM CHANNELS.  Zeshan P. Sheikh , Timothy P. Lynagh, Stephan A. Pless 129-Plat 11:00 am MECHANISM OF NMDA RECEPTOR CHANNEL BLOCK BY MK-801 AND MEMANTINE.  Xianqiang Song , Morten Ø. Jensen, Vishwanath Jogini, Richard A. Stein, Chia-Hsueh Lee, Hassane S. Mchaourab, David E. Shaw, Eric Gouaux 130-Plat 11:15 am MEASURING CONFORMATIONAL DYNAMICS OF amPA RECEPTOR-TARP COMPLEXES USING FRET.  Ljudmila Katchan , Yuchen Hao, Andrew J. Plested 131-Plat 11:30 am MOLECULAR MECHANISMS OF NMDA RECEPTOR FUNCTION AND REGULATION.  Nami Tajima 132-Plat 11:45 am IDENTIFICATION OF NEUROSTEROID BINDING SITES ON GABA A RECEP- TORS USING PHOTOLABELING WITH MASS SPECTROMETRY.  Zi-Wei Chen , John Bracamontes, Wayland WL Cheng, Melissa Budelier, Krishnan Kathiresan, Mingxing Qian, Douglas F. Covey, Alex S. Evers 133-Plat 12:00 pm “DSPER”–THE DEPOLARIZING PROTEIN OF HUMAN SPERM.  Nadine Mundt , Polina Lishko 134-Plat 12:15 pm VISUALIZING ADENINE NUCLEOTIDE REGULATION OF THE K ATP CHANNEL.  Samuel G. Usher , Natascia Vedovato, Michael C. Puljung, Frances M.

F R I D A Y

S U N D A Y

Ashcroft 135-Plat

12:30 pm MECHANISM OF ANION CONDUCTION IN THE CALCIUM-ACTIVATED CHLORIDE CHANNEL TMEM16A.  Andy Lam , Raimund Dutzler

19