Biophysical Society 62nd Annual Meeting | Program Guide

854-Pos Board B624 DECIPHERING THE ROLE OF ATPASE DOMAINS OF CLPA USING SINGLE- MOLECULE OPTICAL TWEEZERS. Hema Chandra Kotamarthi , Robert Sauer, Tania Baker 855-Pos Board B625 MEASURING OLIGONUCLEOTIDE HYBRIDIZATION KINETICS IN SOLUTION USING A TIME-RESOLVED 3D SINGLE-MOLECULE TRACKING TECH- NIQUE. Yuan-I Chen , Cong Liu, Stephanie Phillion, Tim Yeh 856-Pos Board B626 TO FLASH OR NOT TO FLASH? CHARACTERIZATION OF FLUORESCEIN ARSENICAL HAIRPIN (FLASH) AS A PROBE FOR SINGLE-MOLECULE FLUO- RESCENCE SPECTROSCOPY. Dennis D. Fernandes , Jasbir Bamrah, Sent- hilkumar Kailasam, Gregory-Neal W. Gomes, Yuchong Li, Hans-Joachim Wieden, Claudiu C. Gradinaru 857-Pos Board B627 PRECISE MEASUREMENT OF SINGLE-MOLECULE ROTATIONAL DIFFUSIV- ITY IN SOLUTION. Hsiang-Yu Yang , W. E. Moerner Optical Spectroscopy: CD, UV-VIS, Vibrational, Fluorescence (Boards B628–B650) 858-Pos Board B628 MEASURING STRUCTURE AND DISORDER OF (CY3)2 DIMER LABELED DNA FORK-JUNCTIONS USING TWO-DIMENSIONAL FLUORESCENCE SPECTROS- COPY (2DFS). Amr Tamimi , Dylan J. Heussman, Loni M. Kringle, Peter H. von Hippel, Andrew H. Marcus 859-Pos Board B629 PHOTOPHYSICAL BEHAVIOR OF MNEONGREEN, AN EVOLUTIONARY DIS- TANT GREEN FLUORESCENT PROTEIN. Frederik Steiert, Eugene P. Petrov, Petra Schwille, Thomas Weidemann 860-Pos Board B630 RED-SHIFTED FLUORESCENT PROTEINS IMPROVE FRET BIOSENSORS FOR HIGH-THROUGHPUT FLUORESCENCE LIFETIME SCREENING. Tory Schaaf , Ang Li, Benjamin Grant, Prachi Bawaskar, Evan Kleinboehl, Ji Li, Gregory Gillispie, David Thomas 861-Pos Board B631 FRET BASED DETECTION OF MMP-9. Sunil Ajit Shah , Wlodek Mandeki, Ji Li, Zygmunt Gryczynski, Julian Borejdo, Ignacy Gryczynski, Rafal Fudala 862-Pos Board B632 DIRECTIONALITY OF TWO-PHOTON EXCITATION IN REPRESENTATIVE FLUORESCENT PROTEINS. Olga Rybakova , Stepan Timr, Josef Lazar 863-Pos Board B633 HOMOFRET ASSAY FOR DETECTING MICROTUBULE FORMATION BELOW THE DIFFRACTION LIMIT OF LIGHT. Joy Suh, Joseph Beggs, Malena Max- well, Francesca Varias, Keisuke Hasegawa 864-Pos Board B634 MONITORING NATIVE AND AGGREGATE STRUCTURE OF amINO ACIDS AND HUMAN INSULIN WITH BLUE AUTOFLUORESCENCE.  Nathan Cumberbatch , Jillian Madine, Heike Arnolds 865-Pos Board B635 CONCURRENT HOMO- AND HETERO-FRET MEASUREMENTS ENHANCE STUDIES OF PROTEIN INTERACTIONS AND ENABLE DEVELOPMENT OF DUAL BIOSENSORS. Tuan A. Nguyen , Grace Taumoefolau, Youngchan Kim, Henry L. Puhl III, Steven S. Vogel

866-Pos Board B636 USING SPECTRAL PHASOR ANALYSIS OF UV-EXCITED AUTOFLUORES- CENCE TO REVEAL SMALL DIFFERENCES BETWEEN CHEMICALLY-INDUCED MITOCHONDRIAL RESPONSES. Chong Kai Wong, Nazar Al Aayedi, Madhu Gaire, Martin Heidelman, Paul Urayama 867-Pos Board B637 MODULATIONS OF CA 2+ SENSITIZERS AND PHOSPHORYLATION OF CTNT IN DYNAMIC EQUILIBRIUM OF CTNC N-DOMAIN. William Schlecht, Wen-Ji Dong 868-Pos Board B638 SEDIMENTATION BOUNDARY STRUCTURE OF MULTI-COMPONENT SOLU- TIONS WITH RAPIDLY REVERSIBLE INTERACTIONS. Peter Schuck , Sumit K. Chaturvedi, Huaying Zhao 869-Pos Board B639 ACCURATE CD SPECTRUM PREDICTIONS WITH SESCA: INCLUDING PRO- TEIN FLEXIBILITY AND SIDE CHAINS. Gabor Nagy 870-Pos Board B640 SEGMENTAL 13 C-LABELING FOR RAMAN STUDIES OF Α-SYNUCLEIN amY- LOID STRUCTURE IN CELLS.  Jessica D. Flynn , Zhiping Jiang, Shannon M. Lacy, Jennifer C. Lee 871-Pos Board B641 SINGLE-SHOT MICROSECOND-RESOLVED SPECTROSCOPY OF THE BACTERIORHODOPSIN PHOTOCYCLE WITH QUANTUM CASCADE LASER FREQUENCY COMBS. Markus Geiser, Jessica L. Klocke, Markus Mangold, Pitt Allmendinger, Andreas Hugi, Pierre Jouy, Balint Horvath , Jerome Faist, Tilman Kottke 872-Pos Board B642 USING SURFACE ENHANCED RAMAN SPECTROSCOPY TO PROBE SINGLE- CELL NF-KB DYNAMICS DURING TNF-Α STIMULATION. Mamadi M.s Colley 873-Pos Board B643 PROBING PROTEASE ACTIVE SITES WITH VIBRATIONAL REPORTERS.  Meiqi Luo , Christopher N. Eaton, Christine M. Phillips-Piro, Edward E. Fenlon, Scott H. Brewer 874-Pos Board B644 STUDY OF REDOX PROCESS OF CYTOCHROME C IN YEAST UNDER COLD PLASMA IRRADIATION THROUGH RAMAN MICRO-SPECTROSCOPY. Zhu Chen, Jinghua Liu, Qing Huang 875-Pos Board B645 PROTEIN FOLD RECOGNITION BY CIRCULAR DICHROISM SPECTROSCO- PY. András Micsonai , Frank Wien, Judit Kun, Henrietta Vadászi, Matthieu Réfrégiers, József Kardos 876-Pos Board B646 A COARSE-GRAINED MODEL OF CIRCULAR DICHROISM OF PRO- TEINS. Mauricio D. Carbajal-Tinoco , Carmen G. Granados-Ramírez, Claudia G. Benítez-Cardoza 877-Pos Board B647 PROBING LOCAL PROTEIN ENVIRONMENTS WITH A VIBRATIONAL RE- PORTER UNNATURAL amINO ACID. Gwendolyn Fowler , Caroline Kearney, Trexler Hirn, Lukasz Olenginski, Daniyal Tariq, Christine M. Phillips-Piro, Scott H. Brewer 878-Pos Board B648 MICRO- AND NANOSTRUCTURED SURFACE ARCHITECTURES FOR LABEL- FREE INTERROGATION OF PROTEIN STRUCTURE AND FUNCTION.  Julia Flesch , Tabea Brodöl, Maximilian Bettenhausen, Marcin Kazmierczak, Subhajit Guha, Changjiang You, Bernd Witzigmann, Thomas Schroeder, Jacob Piehler

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