Biophysical Society 62nd Annual Meeting | Program Guide

Exhibitor Presentation Bruker Corporation 9:30 am–11:00 am, Exhibit Hall, Room 5

New Member Welcome Coffee 10:15 am–11:15 am, North, Lower Lobby, Room 20/21 Calling all new BPS members! Come and mingle with BPS Staff, Soci- ety Council, and programmembers as you learn about the Society’s activities. Current members are welcome to come and meet with new members. Exhibitor Presentation Dynamic Biosensors GmbH 10:30 am–12:00 pm, Exhibit Hall, Room 6 Biophysical Analysis of Molecular Interactions with the switchSENSE Biosensor switchSENSE is an automated biosensor chip technology employing electrically actuated DNA nanolevers for the real-time measurement of binding kinetics (k ON , k OFF ) and affinities (K D ). nteractions between proteins, DNA/RNA, and small molecules can be detected with femto- molarsensitivity. At the same time, protein diameters (D H ) are analyzed with Angstrom accuracy and conformational changes and melting transitions (T M ) can be measured using minimal amounts of sample. The principles and applicability of static and dynamic measurement modali- ties will be introduced in this talk. We will discuss unique possibilities for the functionalization of the sensor surface, e.g., the adjustment of ligand densities and the precise assembly of different ligands on bifunctional nanolevers. Application examples from fundamental research and drug development will be presented, including: • Introduction to the analysis of molecular interactions with electro- switchable DNA nanolevers • Quantification of conformational changes in proteins and Stokes radius measurements • Analysis of complex binders: high-affinity and bispecific antibody formats • CRISPR/Cas9 – nucleic acid interactions and enzymatic activity measurements • Controlling the density of ligands on a chip surface by electrical desorption and “invisibility cloaking” • TUTORIAL: Programming of measurement workflows and data analysis Speakers Ulrich Rant, CEO, Dynamic Biosensors GmbH Kenneth Dickerson, Director of Business Development in North America, Dynamic Biosensors GmbH Joanna Deek, Scientist, Dynamic Biosensors GmbH Felix Kroener, Scientist, Dynamic Biosensors GmbH Daisylea de Souza Paiva, Technical Sales Manager, Dynamic Biosensors GmbH Symposium Synaptic Vesicle Fusion and Retrieval 10:45 am–12:45 pm, North, Lower Lobby, Room 24 Co-Chairs Axel Brunger, Stanford University Diasynou Fioravante, University of California, Davis 988-Symp 10:45 am MOLECULAR MECHANISMS OF SYNAPTIC VESICLE PRIMING.  Axel Brunger 989-Symp 11:15 am TRANSLATING NEURONAL ACTIVITY AT THE SYNAPSE: THE ROLE OF THE PROTEIN KINASE C CASCADE IN SHORT-TERM PLASTICITY.  Diasynou Fioravante

M O N D A Y

The Latest in Mechanobiology Research with AFM Mechanobiology-related research is focused on understanding how cells exert and respond to forces. Examining the effects of forces on cells has a wide-range of applications from understanding disease pathology to the development of tissue engineering devices. Recent advances in atomic force microscopy (AFM) are not only allowing direct observation of cell membrane structures, such as microvilli, on living cells, they are also pro- viding unique opportunities to measure the nanomechanical properties of individual cells, map the spatial distribution of membrane receptors, as well as study the dynamics of various cellular processes and behaviors. In this session we will introduce the newest advancements in AFM technology designed to enable quantitative nanomechanical property research at the cellular and molecular levels. Come see how researchers can look at, map, and measure mechanical properties like the adhesion forces between cells and molecules, and visualize their dynamic behav- iors, as well as capture high-resolution images. Before you come, check out these leading researcher interviews, talking about their current work using AFM: • Using AFM to study cell mechanics and function: https://www.news- medical.net/news/20171017/Using-AFM-to-study-cell-mechanics- and-function.aspx • Probing living cells with AFM: https://www.news-medical.net/ news/20171121/Probing-living-cells-with-AFM.aspx • Using AFM to study cancer cells: https://www.news-medical.net/ news/20171114/Using-AFM-to-study-cancer-cells.aspx • Studying the nanomechanical properties of aging and cancerous cells using AFM: https://www.news-medical.net/news/20171031/ Studying-the-nanomechanical-properties-of-aging-and-cancerous- cells-using-AFM.aspx • Measuring biological samples using SNAP: https://www.news-medi- cal.net/news/20171024/Measuring-biological-samples-using-SNAP. aspx Speaker Ian Armstrong, Sales Applications Manager, Bruker Corporation Career Development Center Workshop Demystifying the Academic Job Search II: Preparing your Written Application Materials: CV, Cover Letter, and Research Statement 10:00 am–11:00 am, South, Lower Level, Room 2 Over 90% of the cuts in a typical academic job search are made on the basis of your written application materials. Given the large number of candidates in a typical applicant pool, your documents must convey the most important information about you in the most clear and efficient manner. Learn about how your materials should differ based on the type of institution and/or program, and how to create “glance-able” docu- ments to speak most effectively on your behalf. Exhibits 10:00 am–5:00 pm, Exhibit Hall ABC Coffee Break 10:15 am–11:00 am, Exhibit Hall ABC

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