Biophysical Society 62nd Annual Meeting | Program Guide

1269-Pos Board B178 IN VITRO RECONSTRUCTION AND ANALYSIS OF EUKARYOTIC CATALYTIC RIBONUCLEOPROTEIN RNASE P. Anna Perederina, Igor Berezin, Andrey S. Krasilnikov 1270-Pos Board B179 COOPERATIVE RNA RECOGNITION BY A VIRAL TRANSCRIPTION ANTITER- MINATOR. Gonzalo de Prat Gay 1271-Pos Board B180 SOLVENT ACCESSIBILITY OF CRISPR-CAS9 TARGET DNA IS CORRELATED WITH SUBSTRATE SPECIFICITY. Travis H. Hand , Anuska Das, Emily Duboy, Mitchell Roth, Chardasia Smith, Uriel Baptist, Hong Li 1272-Pos Board B181 INTERACTION BETWEEN CAPSID COAT PROTEIN AND MS2 BACTERIO- PHAGE SSRNA WITH DIFFERENT LOOP MOTIF FOR VIRUS ASSEMBLY PROCESS. Lokendra Poudel , Wai-Yim Ching 1273-Pos Board B182 SELECTIVE PACKAGING OF HIV-1 RNA BY GAG PROTEINS.  Ioulia F. Rouzina , Robijn Bruinsma 1274-Pos Board B183 CHARACTERIZING PROTEIN AND RNA INTERACTIONS THAT NUCLEATE THE HIV-1 VIRAL ASSEMBLY. Ugonna Mbaekwe , Pengfei Ding, Michael Board B184 A FLEXIBLE REGION OF PRP8 INTERACTS WITH U5 SNRNA LOOP I AND IS FUNCTIONALLY LINKED TO 5’ SPLICE SITE RECOGNITION. AndrewMacRae , Megan Mayerle, Robert Chalkley, Melissa Jurica 1276-Pos Board B185 INHIBITORY EFFECT OF THE DNA TENSION ON THE CRISPR/CAS9 ACTIVITIES. Suleyman Ucuncuoglu , Kassidy Lundy, Ozgur Sahin 1277-Pos Board B186 SINGLE MOLECULE STUDY OF HRAP1 REGULATED TRF2 BINDING MOTIFS ON TELOMERIC DSDNA. Xiaodan Zhao , Vinod Kumar Vogirala, Meihan Liu, Yu Zhou, Sara Sandin, Jie Yan 1277.1-Pos Board B186.1 DETERMINING NUCLEOCAPSID PROTEIN RECOGNITION OF NON-CANON- ICAL SUBSTRATE HAIRPINS. Henrietta Ehirim , Melanie Dillon, Catherine Volle Chromatin and the Nucleoid I (Boards B187–B206) 1278-Pos Board B187 ARTIFICIAL MODIFICATION OF HISTONES AND THE EFFECT ON EPIGENETICS. Kyrsten M. Thibodeau 1279-Pos Board B188 MAPPING COMBINATORIAL EPIGENETIC MODIFICATIONS AT SINGLE NU- CLEOSOME RESOLUTION.  Jen-Chien Chang , Kazuhide Watanabe, Takashi Umehara, Yuichi Taniguchi, Yuko Sato, Hiroshi Kimura, Akiko Minoda 1280-Pos Board B189 INVESTIGATING THE ROLE OF HISTONE MODIFICATION IN NUCLEOSOME FORMATION BY CAG/CTG REPEATS. Whitli Thomas , Catherine Volle 1281-Pos Board B190 SINGLE MOLECULE OBSERVATION OF CHROMATIN COMPACTION REVEALS THE ROLE OF HISTONE TAILS AND THEIR EPIGENETIC MODIFICA- TIONS. Sohn Byeong-Kwon Summers 1275-Pos

1256-Pos Board B165 ROADBLOCKS BY PROTEIN-MEDIATED DNA LOOPS. Zsuzsanna Vörös , Cristin R. Hendrickson, David Dunlap, Laura Finzi 1257-Pos Board B166 UNCOVERING DYNAMICAL MECHANISMS OF TRANSCRIPTION FACTOR READOUT IN DEVELOPMENT USING OPTOGENETICS. Simon Alamos , Yang Joon Kim, Jordan Y. Xiao, Elizabeth Earley, Hernan G. Garcia 1258-Pos Board B167 DETERMINATION OF MUSCLE-SPECIFIC STRUCTURAL VARIATIONS OF TITIN BASED ON MRNA TRANSCRIPT ANALYSIS. Pabodha Hettige , Uzma Tahir, Kiisa C. Nishikawa, Matthew J. Gage Protein-Nucleic Acid Interactions II (Boards B168–B186.1) 1259-Pos Board B168 Education Travel Awardee INVESTIGATING THE MECHANISM OF DNA RECOGNITION BY A CRISPR- CAS12A NUCLEASE. Wei Jiang 1260-Pos Board B169 DUPLEX DNA DESTABILIZATION BY TYPE V CRISPR-CAS NUCLEASES DURING INTERROGATION OF DNA. Vladimir Mekler , Leonid Minakhin, Konstantin Kuznedelov, Karthik Murugan, Dipali Sashital, Konstantin Board B170 A PAM-INDUCED SIGNALLING ACTIVATES THE COMMUNICATION BE- TWEEN HNH AND RUVC IN CRISPR-CAS9. Giulia Palermo , Clarisse Ricci, Yinglong Miao, Martin Jinek, J. AndrewMcCammon 1262-Pos Board B171 CORRELATED SINGLE MOLECULE TWIST AND FLUORESCENCE MEASURE- MENTS ON CRISPR/CAS SYSTEMS. Pierre Aldag , Julene Madariaga Mar- cos, Inga Songailiene, Felix Kemmerich, Virginijus Siksnys, Ralf Seidel 1263-Pos Board B172 REAL-TIME OBSERVATION OF TARGET SEARCH BY THE CRISPR SURVEIL- LANCE COMPLEX CASCADE. Chaoyou Xue, Yicheng Zhu, Xiangmei Zhang, Yeon-Kyun Shin, Dipali G. Sashital 1264-Pos Board B173 BIOPHYSICAL ANALYSIS OF CAS9–DNA INTERACTIONS AND ENZYMATIC ACTIVITY WITH ELECTRO-SWITCHABLE DNA LAYERS. Felix J. Kroener , Ulrich Rant 1265-Pos Board B174 DNA UNWINDING IS THE PRIMARY DETERMINANT OF CRISPR-CAS9 SPECIFICITY. Shanzhong Gong , Helen H. Yu, Kenneth A. Johnson, David W. Taylor 1266-Pos Board B175 EXPLORING MECHANISMS OF SITE-SPECIFIC DNA CLEAVAGE WITH SINGLE MOLECULE SENSITIVITY. Sadie Piatt , Allen Price, Stephen Parziale, Raquel Ferreira 1267-Pos Board B176 SPATIAL ORGANIZATION AND DYNAMICS OF RNA PROCESSING IN CAULO- BACTER CRESCENTUS . Camille Bayas , Jiarui Wang, Marissa K. Lee, Jared M. Schrader, Lucy Shapiro, W. E. Moerner 1268-Pos Board B177 GLOBAL LANDSCAPES OF PROTEIN-RNA RECOGNITION PROVIDE QUANTI- TATIVE TOOLS TO PREDICT AND ENGINEER SPECIFICITY IN RNA STRUC- TURED ELEMENTS. Faruck Morcos , Qin Zhou, Zachary Campbell Severinov 1261-Pos

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