Biophysical Society 65th Annual Meeting Program Guide

Exhibitor Presentation Oxford Instruments NanoAnalysis 3:30 pm - 4:00 pm Multi-Colour Electron Microscopy: Using Energy Dispersive X-ray Spectrometry to Image and Analyse Biological Samples The visualisation and analysis of life science samples has been a chal- lenge throughout the history of electron microscopy. Biological sample preparation and the absence or addition of contrasting agents often play a key role in the development of imaging methodology. But the signals generated in an electron microscope are mostly underutilised by biolo- gists. While energy dispersive x-ray spectrometry (EDS) has been used in materials science for many decades, sample stability and detector sensi- tivity have prevented a broader adoption in life sciences until recently [1]. Multi-colour electron microscopy (MCEM) combines elemental informa- tion about samples produced using EDS with ultrastructural electron data, providing a powerful and informative imaging technique [2]. MCEM addresses key research topics for the biological electron microsco- pist. What is it, where is it and how much? Using examples from biomedi- cal research, animal cells and tissues, and plant cell biology, this talk will demonstrate how the addition of elemental maps to electron images contributes key information that could be used for a variety of biological imaging applications, such as region of interest profiling or automated segmentation of volumetric data). EDS is not only a powerful imaging tool, providing accurate identification of stains, labels, and ultrastructural features, but it can also be used to conduct analysis on the relative quan- tities of a wide range of elements, providing compositional data on native elements and exogenous features. Speaker Louise Hughes, Product Manager Life Science, Oxford Instruments [1] Pirozzi, N.M., Hoogenboom, J.P. and Giepmans, B.N., 2018. ColorEM: analytical electron microscopy for element-guided identification and imaging of the building blocks of life. Histochemistry and cell biol- ogy, 150(5), pp.509-520. [2] Scotuzzi, M., Kuipers, J., Wensveen, D.I., De Boer, P., Hoogenboom, J.P. and Giepmans, B.N., 2017. Multi-color electron microscopy by element- guided identification of cells, organelles and molecules. Scientific reports, 7(1), pp.1-8. Speed Networking 3:30 pm-5:00 pm Networking is made simple at the Virtual Speed Networking session. Con- nect with many biophysicists (including Biophysical Society committee members) through small group discussions in a short amount of time. Mid-career and more experienced scientists could learn how to get more involved in the Society or network for open positions in their labs. Early career scientists could discuss career goals and challenges, get advice on tenure, or grant writing, or find out how to gain recognition for their work. Graduate students and postdocs could make contacts to find their next position. Pre-registration was required for participation.

Exhibitor Presentation Molecular Devices 4:00 pm - 4:30 pm Streamlining Electrophysiology Data Acquisition and Analysis in Ion Channel Study with Axon pCLAMP 11 Software Axon pCLAMP™ 11 software suite provides ion channel researchers with greater capability in electrophysiology data acquisition and analysis. Recent updates to this include an improved Protocol Editor, which allows the user to more easily create complex experimental protocols with many command stimulations, as well as a new Batch Analysis feature which streamlines analysis of multiple data sets. In this webinar, Dr. Jeffrey Tang will highlight these and other new features in pCLAMP 11 to streamline the data acquisition and analysis in your ion channel study, and how this software package allows you to performmore experiments and obtain more data in your electrophysiology research. Speaker Jeffrey Tang, Senior Application Scientist, Molecular Devices

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