Biophysical Society 65th Annual Meeting Program Guide

Platform Microtubule-associated Motors-Cytoskeleton-based Intracellular Transport 12:00 pm - 1:30 pm Chair Lukas Kapitein, Utrecht University, The Netherlands No Abstract 12:00 pm CYTOSKELETAL DYNAMICS AND KINESIN-1 SELECTIVITY IN DEVELOPING NEURONS. Lukas Kapitein 1390-Plat 12:30 pm THE MITOTIC KINESIN-6 KIF20A IS AN UNCONVENTIONAL TRANSPORTER AND NETWORK BUILDER. Fanomezana M. Ranaivoson , Amna Abdalla Mohammed Khalid, Steven Rosenfeld, Christoph F. Schmidt, Anne M. 12:45 pm LONG-RANGE COUPLING BETWEEN KINESINS REGULATES COLLECTIVE MOTOR BEHAVIOR. Shane A. Fiorenza , Sithara Wijeratne, Meredith D. Betterton, Radhika Subramanian 1392-Plat 1:00 pm ENGINEERED ICOSAHEDRAL PROTEIN CAGE AS A FLEXIBLE MODEL SYSTEM TO STUDY PROPERTIES OF MULTI-MOTOR TRANSPORT OF VIRAL CARGOES. Somaye Badieyan , Michael P. Andreas, Tobias W. Giessen, Michael A. Cianfrocco 1393-Plat 1:15 pm PATHOGENIC MUTATIONS IN THE CHROMOKINESIN KIF22 DISRUPT ANAPHASE CHROMOSOME SEGREGATION. Alex F. Thompson , Patrick R. Blackburn, Jane B. Lian, Eric W. Klee, Jason Stumpff Platform Molecular Dynamics and Bioinformatics III 12:00 pm - 1:30 pm Chair Matthieu Wyart, EPFL, Switzerland 1394-Plat 12:00 pm ARCHITECTURE AND CO-EVOLUTION OF ALLOSTERIC MATERIALS. Matthieu Wyart 1395-Plat 12:30 pm PERMUT - SPATIALLY RESOLVED HYDRATION ENTROPIES FROM ATOMIS- TIC SIMULATIONS. Leonard P. Heinz , Helmut Grubmueller 1396-Plat 12:45 pm GOLEM: AUTOMATED AND ROBUST CRYO-EM-GUIDED LIGAND DOCKING WITH EXPLICIT WATER MOLECULES. Zhiyu Zhao , Emad Tajkhorshid 1397-Plat 1:00 pm REPLICA EXCHANGE MONTE CARLO METHOD FOR PROTON AND X-RAY INDUCED DNA DAMAGE AND REPAIR. Thienbao N. Carpency , Megan Cordone, Camila Quintero Hilsaca, Zachary Condon, Sarah Castro 1398-Plat 1:15 pm GEOMETRIC DEEP LEARNING ON BIOMOLECULAR STRUCTURE. Raphael Townshend , Ligia Melo, David Liu, Ron O. Dror Houdusse 1391-Plat

General Networking 1:00 pm-2:00 pm

Catch up with colleagues and friends in the biophysical community during our networking hour. This event, which is open to all meeting attendees, provides meet-and-greet opportunities to enhance your virtual meeting experience. Exhibitor Presentation Curi Bio 1:30 pm - 2:00 pm Bioengineering the Cell Environment for More Mature and Predictive 2D and 3D Models Cells in the body use a variety of cues (structural, mechanical, elec- trochemical, etc.) from the extracellular matrix (ECM) to develop and mature physiologically. These influential cues help regulate a broad spectrum of processes such as cell signaling, division, and differentiation. Many in vitro platforms seek to combine and incorporate these cues into the cell’s microenvironment but often fail, suffering from lack of reproducibility and incompatibility with other well-established end-point assays. Here, we demonstrate biomimetic in vitro platforms capable of reliably reproducing these essential cues. These platforms markedly improve the structural and functional development of a variety of cell types, including stem cells, cardiomyocytes, muscle cells, and more. These cutting-edge strategies can be deployed in both 2D and 3D model systems for high- throughput assessment of metabolism, electrophysiology, and contractil- ity. We describe how the differentiation of stem cells can be enhanced by providing a more biomimetic culture environment, with a particular focus on iPSC-derived cardiomyocytes and skeletal muscle cells. Further, we highlight how an active mechanical environment combined with aligned cell-nanotopography cues improves adhesion, signaling, and polarity across many cell applications. Finally, we demonstrate how to scale these technologies to 3D organoid systems, and how these approaches can improve the development of in vitro disease models to support the discovery of new therapies. Speaker Hamed Ghazizadeh, Product Manager, Curi Bio Break 1:30 pm - 2:00 pm Poster Presentations and Late Posters 2:00 pm - 3:30 pm

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