Biophysical Society Bulletin | January 2024

Sponsored Content

Nicoya Alto digital SPR

Alto™ is the world’s first and only SPR platform to integrate DMF with nanotechnology-based biosensors, providing a revolutionary approach to real-time interaction analysis. Many common assay methods are automated using Alto’s soft ware, the Nicosystem, and executed within Alto’s self-contained cartridge. Small sample droplets are dispensed, serially diluted, and transported to the sensor surface to determine association kinetics, where they are moved back-and-forth to achieve strong mixing. To determine dissociation kinetics, bu er is transported to the sensor surface and moved back-and-forth. By automating and miniaturizing assays, Alto takes the complexity out of SPR, while equipping your pipeline with a solution that is:

Sample-friendly: Get full kinetics from 2 µL of purified or crude sample, 100-200X less than traditional SPR platforms.

User-friendly: Spend 70% less time at the bench with Alto’s automated sample manipulation and seamless user interface.

Budget-friendly: Minimize lab downtime and maintenance costs with Alto’s fluidics-free operation.

How DMF improves SPR

Minimizes impacts of depletion and accumulation: by using 30X less surface area than conventional SPR platforms, rapid mixing rates, and multiple droplet cycles. Lowers signal artifacts: through DMF-based delivery of samples to sensors. Instead of buffer blank subtraction, Alto uses a detrend ing algorithm to correct for any baseline drift.

Increases precision and accuracy: through automated sample manipulation. Alto demon strated superior precision and accuracy against mechanical pipettes and liquid handlers. Decouples interaction time from sample volume: with droplet

oscillation that enables unlimited sample interaction time without any sample dispersion.

Interested in learning more about Alto digital SPR? Contact us today!

Reduces mass transport limitations: by lower ing the diffusion boundary layer to be equiva lent to fast flow rates and confining the detec tion range to use lower amounts of ligand.

January 2024

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