Biophysical Society Conference | Estes Park 2023

Membrane Budding and Fusion

Poster Abstracts

37-POS Board 13 ESCRT MACHINERY AND ATG PROTEINS COORDINATE TO REPAIR DAMAGED ORGANELLES

Kevin P Bohannon 1 ; Kimberly A Edicha 1 ; Jiaqian Li 1 ; Wei Chen 1 ; Phyllis I Hanson 1 ; 1 University of Michigan, Biological Chemistry, Ann Arbor, MI, USA

Endosomal Sorting Complex Required for Transport (ESCRT) and Autophagy-related (ATG) proteins play instrumental roles in establishing and preserving compartmentalized spaces within eukaryotic cells. Previous studies, including our own, have demonstrated that ESCRT machinery contributes to the repair of organelles with disrupted membranes, yet the exact processes behind this function remain elusive. In this study, we report that in the absence of numerous ATG proteins, the ESCRT machinery is unable to repair damaged lysosomes and phagosomes, worsening small molecule leakage from these compartments. We highlight this interdependence by monitoring the translocation of individual ATG and ESCRT machinery components. Our findings indicate a mutual reliance of the ESCRT and ATG systems for effective membrane repair, underscoring the importance of understanding the exact molecular signals that trigger the recruitment of the membrane repair machinery.

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