Biophysical Society Thematic Meeting | Ascona 2026

Mechanobiology of Infection

Poster Abstracts

13-POS Board 13 HIGH-SENSITIVITY NUCLEIC ACID CRYPTIC LOCK ELECTROCHEMICAL SENSOR: PORTABLE PLATFORM ENABLING POCT Yichen Liu ; Jia Geng Sichuan University, West China School of Medicine, Chengdu, China Nucleic acid detection is a cornerstone technology for rapid clinical diagnosis and point-of-care testing (POCT). Compared to fluorescence-based methods, electrochemical nucleic acid detection offers superior advantages for the miniaturization and portability of instrumentation, garnering significant research interest in recent years. Although researchers have developed several molecular recognition pathways with promising industrialization potential through the optimization of electrochemical probes, challenges remain in achieving comparable detection sensitivity and speed. To overcome this bottleneck and enable multiplexed, precise electrochemical detection of nucleic acid targets, we innovatively propose a one-pot, rapid detection method based on a sealed PGE-LAMP electrochemical chip. The core of this methodology lies in the precise modulation of the binding free energy of 3-way/4-way electrochemical nucleic acid probes (Nucleic Acid Cryptic Locks). This enables specific target binding to unlock the cryptic locks, thereby releasing electrochemical signal probes. The released probes subsequently hybridize with the P-Acceptor strand immobilized on the electrode surface, and the reaction is monitored in real-time by scanning for characteristic oxidation peaks using Square Wave Voltammetry (SWV). This method demonstrates high sensitivity, specificity, and a superior signal-to-noise ratio. Furthermore, we have developed a companion portable electrochemical detection instrument, laying a solid foundation for the practical bedside application of this technology and facilitating the broader implementation of LAMP technology.

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