Biophysical Society Thematic Meeting | Ascona, Switzerland

Liposomes, Exosomes, and Virosomes: From Modeling Complex Membrane Processes to Medical Diagnostics and Drug Delivery

Poster Abstracts

18-POS Board 9 Investigation of the Use of Exosomes as Drug Delivery Systems Ellie Barlow Myers, Salomé Guillaumin, Jean-Marie Devoisselle, Joel Chopineau , Marie Morille. n/a, , France. Exosomes, as natural vectors of biomolecules, has been described as interesting drug delivery systems for mainly nucleic acids, but also lipids or proteins. We propose to use pharmaceutical and chemical methods (post-insertion, click chemistry,…) to allow drug loading as well as surface modification to increase the pharmacokinetics behavior and targeting efficiency with “easy to handle” processes which should facilitate the development of new exosome-based therapeutics. The efficient exosome-mediated delivery in vivo requires targeting vesicles for uptake by specific recipient cells, as the half life of non targeted exosomes was close to 2min after systemic administration in mice (Morishita et al., 2015; Smyth et al., 2015; Wiklander et al., 2015). As a supplementary hurdle, frequently encountered targeting process used to modify exosomes, require transfection of cells and lead to low production yield (Alvarez-Erviti et al., 2011; Hung et al., 2015). In this context, we choose to modify human mesenchymal stem cells derived exosomes in a post-production manner, by various physico-chemical methods. We are currently working on the surface modification of exosomes (PEGylation, ligand attachment). These modifications are assessed by physico-chemical characterization (size, surface charge), and quantification of the grafted molecules (PEG quantification (KI/I2), ELISA,…) at the exosome surface. In parallel, the loading ability is evaluated for different kind of molecules (chemicals, protein, nucleic acids), and different process. The efficiency of surface modification will be evaluated in vitro in regards to interaction with plasma proteins as well as with immune cells (THP1 monocytes) thanks to a fluorescent tracking (FACS, confocal microscopy). The interaction with cells which produced exosomes and other cell lineage (tumoral cell line) will be evaluated.

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