Biophysical Society Thematic Meeting | Ascona, Switzerland

Liposomes, Exosomes, and Virosomes: From Modeling Complex Membrane Processes to Medical Diagnostics and Drug Delivery

Poster Abstracts

48-POS Board 24 Polymer-Supported Lipid Bilayers Derived from Native Cell Membranes as Platforms for Studying Biological Nanoparticle-Cell Interactions Hudson Pace , Nadia Peerboom, Eneas Schmidt, Virginia Claudio, Marta Bally, Fredrik Höök. Chalmers University of Technology, Göteborg, Sweden. The ability to produce polymer-supported lipid bilayers (pSLBs) that contain native membrane components which retain transmembrane protein mobility and activity offers a new paradigm in the study of nanoparticle-cell surface interactions. These pSLBs are created using native cell membrane vesicles (NMVs) (i.e., plasma membrane preparations or ghost cells) which allows these biomimetic surfaces to display the complex composition of the plasma membrane in a format amenable to a wide range of surface-sensitive analytical techniques. Characterization of the mobility and functionality of membrane proteins contained within these pSLBs will be discussed. Additionally, compositional and physical characterization of the NMVs will be presented. The utility of this platform will be presented in the context of two systems: 1) the study of Herpes Simplex Virus (HSV) interactions with the pSLBs derived from monkey kidney (VERO) cells and 2) the study of exosomes isolated from glioma cell cultures and their interactions with pSLBs derived from endothelial cells. The HSV/VERO system was used in investigating the potency of viral-binding inhibitors, while the exosome/endothelial system was used to investigate the role of exosomes in angiogenesis. Total Internal Reflection Microscopy (TIRFM) in combination with single-particle tracking of fluorescently labeled biological nanoparticles provided the ability to investigate single-particle binding kinetics in both systems. These systems illustrate the potential of single-particle tracking on NMV-derived pSLBs for the comprehensive investigation of the interaction characteristics of individual biological nanoparticles with native cell membranes.

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