Biophysical Society Thematic Meeting | Ascona, Switzerland

Liposomes, Exosomes, and Virosomes: From Modeling Complex Membrane Processes to Medical Diagnostics and Drug Delivery Board 10 Monodisperse Multicompartment Liposomes Nan-Nan Deng , Wilhelm Huck. Radboud University, Nijmegen, Netherlands. 19-POS

Poster Abstracts

Liposomes are self-assembled phospholipid vesicles with great potential in fields ranging from drug delivery to synthetic biology. However, high throughput, controlled fabrication of monodisperse liposomes with controllable compartments has not been achieved thus far. Here, we report on a surfactant-assisted microfluidic route to assemble oil-free monodisperse liposomes with single- or multi-compartments via precise control over interfacial energies of complex water-in-oil-in-water (W/O/W) emulsion systems. The use of various emulsion templates, in combination with the spontaneous dewetting afforded by the surfactant, allows for the creation of uniform, single bilayer liposomes with multiple inner compartments. This method is compatible with complex bioreactions as exemplified by the production of eGFP in liposomes via cell-free gene expression. We believe this is the example where liposome formation from microfluidically prepared double emulsion templates is fully controlled. The high yield, high throughput, and uniformity of structures produced has potential for future designs of cell-like bio-devices or as coupled microreactors.

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