Biophysical Society Thematic Meeting | Ascona, Switzerland

Liposomes, Exosomes, and Virosomes: From Modeling Complex Membrane Processes to Medical Diagnostics and Drug Delivery

Poster Abstracts

25-POS Board 13 G Protein-Coupled Receptors Incorporated Into Rehydrated Diblock Copolymer Vesicles Retain Functionality Mary Gertrude Gutierrez 1 , Farzad Jalali-Yazdi 1 , Justin Peruzzi 2 , Carson T. Riche 1 , Richard W. Roberts 1 , Noah Malmstadt 1 . 1 University of Southern California, Los Angeles, USA, 2 University of Virginia, Charlottesville, VA, USA. We successfully incorporated the human serotonin receptor, G Protein Coupled Receptor (GPCR), 5-HT 1A R, in polymeric giant unilamellar protein-vesicles (pGUPs) made of diblock copolymer. By utilizing an agarose rehydration technique for protein incorporation, the GPCR is inserted in the biased, physiological orientation with the C-terminus cytosolic and N-terminus extracellular. The GPCR is fully functional within the polymeric bilayer, exhibiting physiological responses to various ligands. The entire population of GPCRs in pGUPs remains fully functional after lyophilization for 120 hours.

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