Biophysical Society Thematic Meeting| Les Houches 2019

Multiscale Modeling of Chromatin: Bridging Experiment with Theory

Poster Abstracts

21-POS Board 21 A THEORETICAL STUDY OF INHERITANCE OF NUCLEOSOME POSITIONING DURING DNA REPLICATION Tripti Bameta 3 ; Dibyendu Das 2 ; Ranjith Padinhateeri 1 ; 1 Indian Institute of Technology Bombay, Biosciences & Bioengineering, Mumbai, Maharashtra, India 2 Indian Institute of Technology Bombay, Physics, Mumbai, Maharashtra, India 3 UM-DAE Centre for Excellence in Basic Sciences, , Mumbai, Maharashtra, India experimentally known facts, we propose a model to study nucleosome reorganization during DNA replication. Our model has four kinetic events, namely, replication fork movement, nucleosome disassembly ahead of the fork, nucleosome deposition behind the fork and ATP- dependent repositioning of nucleosomes behind the fork. Simulating nucleosome re-organization during replication, we argue that short pausing of replication fork, associated with nucleosome disassembly, can be an event crucial for communicating nucleosome positioning from mother chromatin to daughter chromatin. Our simulations obtain de novo nucleosome assembly similar to what is observed in experiments and predict regions in parameter space where nucleosome positioning may be inherited during DNA replication. Nucleosome positioning is crucial for many cellular processes. How nucleosome positioning is inherited from mother chromatin to daughter is poorly understood. Accounting for

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