Biophysical Society Thematic Meeting - November 16-20, 2015

Biophysics in the Understanding, Diagnosis, and Treatment of Infectious Diseases Poster Abstracts

14-POS Board 14 Blood Levels of 8-Isoprostane F2 Alpha in Chronic Hepatitis C Soad Eltabakh , Fathalla Ismael, Hanan Abdelaziz, Hala Maged, Rasha Ghazala. Alexandria University, Alexandria, Egypt. BACKGROUND/PURPOSE: The mechanism by which HCV causes liver damage is mediated through immunological means, direct viral toxicity and induction of oxidation stress (OS) . 8- Isoprostane F2 Alpha (8-Isop) is an important marker to assess the OS in vivo and used as extensively to qualify lipid peroxidation. The purpose of the study was to determine the relation of 8-Isop to the severity of HCV related liver diseases. METHODS: Fifty subjects were evaluated and divided into 5 groups, 10 in each group : G1 : Chronic hepatitis C without cirrhosis. G2 : Chronic hepatitis C with child A cirrhosis. G3 : Chronic hepatitis C with child B cirrhosis. G4 : Chronic hepatitis C with child C cirrhosis. G5 : Volunteers with no evidence or history of liver disease .All subjected to : determination of 8-Isop and lipid profile. RESULTS: Statistical comparison between the mean value of 8-Isop in the studied groups using the F test showed significant increase in groups 2, 3, and 4 than in G5. Also there was significant increase of 8-Isop in G1 than G2, and in G2 than G3, also, in G3 than G4. On the other hand, there was significant association between the severity of HCV liver disease and low cholesterol, TG, LDL, and VLDL. COCLUSION: This results highlight the importance of OS , marked by 8-Isop in the pathogenesis and severity of HCV related liver disease. Also, the findings reported the importance of lipid profile parameters and its relation to disease severity. Further studies are needed entailing the association between lipid levels, liver histo-pathological characters, and virological parameters to predict the response to the therapy. Reference: Spengler U, Nattermann J. Immunopathogenesis in hepatitis C virus cirrhosis.Clin Sic (London) 2007; 112(3):141-155.

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