Biophysical Society Thematic Meeting| Padova 2019

Quantitative Aspects of Membrane Fusion and Fission

Poster Abstracts

18-POS Board 18 THE INTERACTIONS BETWEEN THE NUCLEAR PORE COMPLEX AND THE PORE MEMBRANE (POM) REGION WITHIN THE NUCLEAR ENVELOPE Joseph S Glavy 1 ; 1 University of Texas at Tyler, Pharm. Sci, Tyler, Texas, USA The nuclear envelope separates into three domains: the outer nuclear membrane (ONM), the inner nuclear membrane (INM) and the pore membrane (POM). The POM is the region of the nuclear envelope where INM and ONM merge occurs. The POM curvatures are coated with select membrane proteins believed to anchor Nuclear Pore Complexes (NPCs) like the prongs of a diamond ring. POM proteins are involved in the initiation of pore complex construction, stabilization, emancipation and eventual reformation of the NPC. The three-dimensional structure of purified complexes was determined by single particle electron microscopy. To we uncover their dynamic interacting layers of the NPC including areas within POM curvatures, we crosslinked the engaged complexes within the human NPC (hNPC) and the subsequent identification of crosslinked peptides through crosslinking mass spectrometry (XL-MS), These data, along with a high resolution structure of the hNPC were integrated to determine how the layers of the hNPC come together forming encrusted rings and their connectivity to the POM curvatures.

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