Biophysical Society Thematic Meeting| Padova 2019

Quantitative Aspects of Membrane Fusion and Fission

Friday Speaker Abstracts

THE POWER OF CORRELATIVE SUPER-RESOLUTION IMAGING Aleksandra Radenovic 1,2 ; 1 EPFL School of Engineering, Lausanne, Switzerland

2 Institute of Bioengineering, Laboratory of Nanoscale Biology1015, Lausanne, Switzerland In this talk I will review recent progress in correlative super-resolution imaging. First part of the talk will be dedicated to our efforts to build and characterize a correlated single molecule localization microscope- with an atomic force microscope (SMLM)/AFM that allows localizing specific, labelled proteins within high-resolution AFM images in a biologically relevant context. The technique has been successfully applied to the live and fixed samples. The second part of my talk will be dedicated to the discussion on the complementarity between SMLM and super- resolution optical fluctuation imaging (SOFI). References High resolution correlative microscopy: Bridging the gap between Single Molecule Localization Microscopy and Atomic Force Microscopy Pascal D. Odermatt, Arun Shivanandan, Hendrik Deschout, Radek Jankele, Adrian, P. Nievergelt, Lely Feletti, Michael W Davidson, Aleksandra Radenovic and Georg E. Fantner, Nano Lett. 15 (8), pp 4896–4904 2015. Complementarity of PALM and SOFI for super-resolution live cell imaging of focal adhesions H. Deschout, T. Lukes, A. Sharipov, D. Szlag, L. Feletti, W. Vandenberg, P. Dedecker, J. Hofkens, M. Leutenegger, T. Lasser, A. Radenovic accepted Nature Communications 2016

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