Biophysical Society Thematic Meeting | Tutzing 2026

Single-Molecule FRET: The Next 30 Years

Poster Abstracts

60-POS

Board 30

EXPANDING THE FRET TOOLBOX WITH SPAD ARRAYS Evangelos Sisamakis 1 ; Max Tillmann 1 ; Anders Barth 1 ; Fabio Barachati 1 ; Marcelle Koenig 1 ; Matthias Patting 1 ; Felix Koberling 1 ; Rainer Erdmann 1 ; 1 PIcoQuant, Berlin, Germany Confocal microscopy is an essential tool in many academic disciplines due to its intrinsic sectioning capability. It combines naturally with time-resolved single photon detection and time correlated single photon counting (TCSPC) approaches. This has established it as the leading platform for time-resolved investigation methods such as fluorescence lifetime imaging (FLIM) and fluorescence correlation spectroscopy (FCS). Recently, high-performance SPAD-arrays featuring few tens of pixels have become available. Combining these with suitable multi-channel TCSPC devices opens up new possibilities in confocal time-resolved sensing. In this work we present the two possible new directions: a) a new algorithm for ISM-FLIM and its application to monitoring protein interactions via FRET with high resolution and contrast , b) a new algorithm for studying diffusion modi in complex environments. without the need of extra calibration measurements.

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