Emerging Concepts in Ion Channel Biophysics

Emerging Concepts in Ion Channel Biophysics

Wednesday Speaker Abstracts

High-Speed Atomic Force Microscopy (HS-AFM): A New Tool for the Direct Study of Conformational Changes in Gated Ion Channels Martina Rangl 1,2 , Yi Ruan 2 , Arin Marchesi 2 , Pierre-Jean Corringer 3 , Crina Nimigean 1 , Simon Scheuring 1,2 . 1 Weill Cornell Medicine, New York, NY, USA, 2 INSERM, Marseille, France, 3 Institut Pasteur, CNRS, Paris, France. The advent of high-speed atomic force microscopy (HS-AFM1) has opened a novel research field for the dynamic analysis of single bio-molecules: Molecular motor dynamics2,3 membrane protein diffusion4, assembly5 and conformational changes of transporters6 could be directly visualized. Further developments for buffer exchange7 and temperature control8 during HS- AFM operation provide breakthroughs towards the performance of dynamic structural biochemistry using HS-AFM. Here, we show the direct visualization of conformational changes of the cyclic nucleotide gated potassium channels upon ligand binding9, and of a pentameric receptor ion channel in pH-gating10. 1) Ando et al., Chemical Reviews, 2014, 114(6):3120-88. 2) Kodera et al., Nature, 2010, 468(7320):72-6. 3) Uchihashi et al., Science, 2011, 333(6043):755-8. 4) Casuso et al., Nature Nanotechnology, 2012, 7(8):525-9. 5) Chiaruttini et al., Cell, 2015,163(4):866-79.

6) Ruan et al., PNAS, 2017, doi: 10.1073/pnas.1616413114 7) Miyagi et al., Nature Nanotechnology 2016, 11: 783-790 8) Takahashi et al., Small, 2016, 12(44):6106-6113 9) Rangl et al., Nature Communications, 2016, 7: doi:10.1038/ncomms12789 10) Ruan et al., in preparation

25