Emerging Concepts in Ion Channel Biophysics

Emerging Concepts in Ion Channel Biophysics

Poster Abstracts

2-POS Board 2 Subcellular localization of Zip3 and ZnT3 Zinc Transporters in Mouse Spermatogenic Cells Karla Lisette Andrade López , Ignacio López González, Julio César Chávez Zamora, Paulina The physiological relevance of the micronutrient zinc during spermatogenesis has been reported in different mammalians. Zinc ions are involved in the spermatogenic cells (SCs) growth and development, chromatin condensation and modulation of oxidative stress in sperm and structural maintenance of seminiferous tubules. Zinc deficiency reduces testosterone production, which is a fundamental hormone for spermatogenesis; increases oxidative stress, seminiferous tubules atrophy and inhibits spermatids differentiation. On the other hand, an excess of zinc has detrimental effects on spermatogenesis, such as a decrease on sperm motility, arrest of SCs and seminiferous tubules atrophy. Therefore, zinc homeostasis must be strictly regulated. To maintain this homeostasis, two different types of zinc transporters are involved, the Zip and ZnT families. The Zip family is composed of fourteen members involve in the entrance of zinc into the cell. Meanwhile, the ZnT family include ten members responsible removing zinc from the cytoplasm. In spite of the zinc physiological relevance in mouse SCs, little is known about the subcellular distribution and the expression of zinc transporters in the different stages of mouse spermatogenesis. In the present report, we immunodetected the presence of Zip3 and ZnT3 transporters in SCs. Our preliminary results suggest that Zip3 transport is mainly located at the plasma membrane of different SCs types. On the contrary, ZnT3 distribution is preferentially express in cytoplasm and intracellular organelles. Consistently with the Zip3 expression in the plasma membrane we observed that extracellular addition of HCO3 [25 mM], a Zip3 modulator, and ZnSO4 [100 µM] increased of intracellular zinc concentration in Fluozin-1-loaded SCs. In conclusion, our preliminary results indicate the expression, at least, of Zip3 and ZnT3 zinc transports in mouse SCs. Acknowledgements: This work was supported by DGAPA/UNAM: IN204914 to ILG, IN203116 to CT. Torres Rodríguez, Claudia L. Treviño Santa Cruz. Biotechnology Institute, Cuernavaca, Mexico.

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