Engineering Approaches to Biomolecular Motors

Engineering Approaches to Biomolecular Motors: From in vitro to in vivo Poster Abstracts

9-POS Board 9 Bio-functionalized Vesicles for the Measurement of Biological Activities and their Automated Generation Huisoo Jang, Sunhee Yoon, Sun Min Kim. Tae-Joon Jeon , Inha University, Incheon, South Korea. We have functionalized polymeric vesicles with antibodies to measure their activities to the target molecules. Polymeric vesicles are made of polydiacetylene that has chromatic transition from blue to red upon external stimuli, such as pH, heat, and perturbation of its structure. These vesicles are further encapsulated within hydrogel matrix to achieve more enhanced biological activities. This process is fully automated using a microfluidic device. The target molecule is phosphinothricin acetyltransferase (PAT) that is one of most abundant protein in genetically modified organisms (GMOs). To recognize PAT, anti-PAT antibody was conjugated onto the surface of the PDA vesicles using EDC/NHS coupled reaction. PDA vesicles were encapsulated into the poly(ethylene) glycol diacrylate (PEG-DA) hydrogel at high concentration to achieve amplified the signal of color. Moreover, we generated a bead type hydrogel using a microfluidic device in an automated manner. With our system, we were successfully able to detect PAT protein in a very low concentration with amplified singals.

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