Program Book

3022-P os B oard #B714 CRYO-EM STUDIES OF DRP1 SELF-ASSEMBLY PROVIDE INSIGHTS INTO THE MECHANISM OF MITOCHONDRIAL FISSION. Frances JD Alvarez, Christopher A. Francy, Jason A. Mears 3023-P os B oard #B715 CRYO-TOMOGRAPHY OF VITRIFIED BACTERIAL AND HUMAN CELLS BY SCANNING TRANSMISSION ELECTRON MICROSCOPY. Sharon G. Wolf , Lothar Houben, Michael Elbaum 3024-P os B oard #B716 COMPRESSED SENSING METHODS FOR ELECTRON TOMOGRAPHY OF CELLULAR STRUCTURE. Matthew D. Guay, Wojciech Czaja, Richard D. Leapman 3025-P os B oard #B717 HELICAL ORGANIZATION OF COAGULATION FACTOR VIII ON LIPID NANOTUBES. Svetla Stoilova-McPhie , Jaimy Miller, Daniela Dalm, Kirill Grushin 3026-P os B oard #B718 ANSWERING REAL BIOLOGICAL QUESTIONS BY COMBINING CRYO-TEM, XRD AND NMR. Eric Hnath , Marc Storms, Jeff Lengyel, Thomas Wohlfarth 3027-P os B oard #B719 ZERNIKE PHASE-CONTRAST ELECTRON TOMOGRAPHY OF MICROTUBULE-RELATED COMPLEXES IN AXONEMES. Haixin Sui , Radostin Danev, Rebecca Fisher, Jie He, Chyongere Hsie, Michael Marko 3028-P os B oard #B720 FLEXIBLE FABS IN THE REFINEMENT OF COMPLEXES BY RESOLVING THE STRUCTURAL BASIS OF FACTOR VIII ACTIVATION. Daniela Dalm , Kirill Grushin, Alexey Y. Koyfman, Jaimy Miller, Svetla Stoilova-McPhie 3030-P os B oard #B722 OPENING WINDOWS INTO THE CELL: FOCUSED-ION-BEAM MILLING FOR CRYO-ELECTRON TOMOGRAPHY. Elizabeth Villa , Miroslava Schaffer, Jürgen M. Plitzko, Wolfgang Baumeister 3031-P os B oard #B723 CRYO-EM ATOMIC MODEL OF BROME MOSAIC VIRUS DE- RIVED FROM DIRECT ELECTRON DETECTION IMAGES AND A REAL-SPACE MODEL OPTIMIZATION PROTOCOL. Zhao Wang , Corey Hryc, Benjamin Bammes, Pavel Afonine, Joanita Jakana, Dong-Hua Chen, Xiangan Liu, Matthew L. Baker, Cheng Kao, Steve J. Ludtke, Michael F. Schmid, Paul Adams, Wah Chiu 3032-P os B oard #B724 STRUCTURAL VISUALIZATION OF MITOTIC CYCLE BY THREE-DIMENSIONAL FOCUSED ION BEAM-SCANNING ELECTRON MICROSCOPE (FIB-SEM) WITH NANOSCALE RESOLUTION AT WHOLE CELL LEVEL. Rina Nagai, Keisuke Ohta, Atsuko H. Iwane 3033-P os B oard #B725 OVERCOMING PATCH-POTENTIAL EFFECTS ON THE SURFACES OF TEM PHASE-CONTRAST DEVICES. Robert M. Glaeser 3034-P os B oard #B726 SUBSTRATE EFFECTS ON STRUCTURAL STUDIES USING CRYO-EM. Lige Tonggu , Liguo Wang SINGLE-PARTICLE TRANSMISSION ELECTRON MICROSCOPY. Ryan MB Hoffman , Andrew B. Ward 3029-P os B oard #B721

3035-P os B oard #B727 A FIRST LOOK INTO THE 3D STRUCTURE OF THE TRPV2 CHANNEL BY SINGLE-PARTICLE CRYO-EM. Guizhen Fan , Jennifer Gonzalez, Olga B. Popova, Theodore G. Wensel, Irina I. Serysheva 3036-P os B oard #B728 A CRYO-EM STRUCTURE OF A MINIMAL TRANSLATION INITIATION SYSTEM: DELETION IN SELF-SUFFICIENT IRES PREVENTS TRANSLOCATION. Amy Jobe , Marisa D. Ruehle, Jeffrey S. Kieft, Joachim Frank 3037-P os B oard #B729 SERIAL BLOCK-FACE SCANNING ELECTRON MICROSCOPY FOR NANOSCALE CHARACTERIZATION OF TISSUE ULTRA- STRUCTURE. Charlotte R. Pfeifer, Andre Shomorony, Guofeng Zhang, Maria A. Aronova, Richard D. Leapman 3038-P os B oard #B730 EXAMINING DRP1 CONFORMATIONAL CHANGES AND DOMAIN INTERACTIONS IN THE MITOCHONDRIAL FISSION COMPLEX USING CRYO-EM. Christopher A. Francy , Chris Frölich, Oliver Daumke, Jason A. Mears 3039-P os B oard #B731 STRUCTURE DETERMINATION OF SMALL MACROMOLECULAR COMPLEXES BY CRYO-ELECTRON MICROSCOPY. Alberto Bartesaghi, Jason Pierson , Prashant Rao, Soojay Banerjee, Mario Borgnia, Lingbo Yu, Lesley Earl, Michael Alink, Jacqueline Milne, Sriram Subramaniam Optical Microscopy and Super Resolution Imaging III (Boards #B732–#B761) 3040-P os B oard #B732 EXPLOITING BINDING KINETICS OF FLUOROGEN ACTIVATING PEPTIDES TO ENHANCE PHOTOSTABILITY: APPLICATIONS TO LIVE CELL SINGLE MOLECULE IMAGING. Saumya Saurabh , Victor R. Mann, Lauren E. Beck, Ming Zhang, Andrea Costello, Marcel P. Bruchez 3041-P os B oard #B733 QUANTITATIVE RETARDANCE IMAGING USING QUADRI- WAVE LATERAL SHEARING INTERFEROMETRY (QWLSI). Sherazade Aknoun , Pierre Bon, Julien Savatier, Benoit Wattellier, Serge Monneret 3042-P os B oard #B734 QUANTIFICATION OF COLOCALISATION; CO-OCCURRENCE, CORRELATION, EMPTY VOXELS, REGIONS OF INTEREST AND THRESHOLDING. Jeremy Adler, Ingela Parmryd 3043-P os B oard #B735 NANOSCALE PROTEIN DIFFUSION BY STED-BASED SPATIOTEMPORAL FLUORESCENCE CORRELATION SPECTROSCOPY. Paolo Bianchini , Francesco Cardarelli, Mariagrazia Di Luca, Alberto Diaspro, Ranieri Bizzarri 3044-P os B oard #B736 MOUSE RETINA IMAGING BY MEANS OF INVERTED SELECTIVE PLANE ILLUMINATION MICROSCOPY (ISPIM). Zeno Lavagnino , Francesca Cella Zanacchi, Luca Lanzanò, Alberto Diaspro 3045-P os B oard #B737 TOMOGRAPHIC INCOHERENT PHASE IMAGING, A DIFFRACTION TOMOGRAPHY ALTERNATIVE. Sherazade Aknoun, Benoit Wattellier , Pierre Bon, Serge Monneret

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Biophysical Society 58 th Annual Meeting, San Francisco, California