Program Book

fluid under physiological conditions. In this tutorial, Bruker will present theories behind AFM, bio-applications in high-speed AFM, and practical guides to quantitative mechanical measurements and analysis of biologi- cal samples ranging from a single membrane protein to a single cell. While the key experiments presented will encompass research in micro- biology/pain mediation/cancer, the methodology has also been employed in other disciplines including pathogenesis/stem cell differentiation/cell signaling and more. Presenter: Senli Guo, Application Scientist, Bruker Nano Surfaces 4:00 pm –5:00 pm , R oom 122 Membership Committee Meeting 4:00 pm –5:00 pm , R oom 300 Career Center Workshop Ten Tough Industrial Interview Questions (and Ten Pretty Good Responses) You’ve been invited to interview with that drug development company that you’ve always wanted to work for. You’ve soaked up the details of the position description. You are confident in your ability to do the job, as well as answer any/all technical questions during the interview process. The day is yours…until…that first question catches you by surprise and your confidence begins to wilt. Be prepared for those non-technical ques- tions that you will almost certainly hear at some point, know why they are asked, and learn what a good (if not great) response to each question might be by attending this workshop. 4:00 pm –6:00 pm , R oom 134 Symposium Future of Biophysics Burroughs Wellcome Fund Symposium Chair Robert Nakamoto, University of Virginia Health Science Center N o A bstract 4:00 pm “OFF-LABEL” USES OF SEQUENCING TECHNOLOGY TO EXPLORE THE PHYSICAL GENOME. William J. Greenleaf N o A bstract 4:30 pm SPHINGOLIPID AND CHOLESTEROL DISTRIBUTION IN THE PLASMA MEMBRANE BY HIGH-RESOLUTION SIMS. Mary L. Kraft N o A bstract 5:00 pm THE INFLUENCE OF EXTRINSIC FLUCTUATIONS ON THE DECISION OF CELLS. Elijah Roberts N o A bstract 5:30 pm HOW CAN LABILE BONDS LEAD TO TOUGHER NETWORKS? THE UNEXPECTED ROLE OF CROSSLINKER KINETICS IN DETERMINING CYTOSKELETAL MECHANICS. Megan T. Valentine 4:00 pm –6:00 pm , R oom 135 Symposium Molecular Basis of Voltage Dependence Co-Chairs Sudha Chakrapani, Case Western Reserve University Eduardo Perozo, University of Chicago 1218-S ymp 4:00 pm THERMODYNAMIC ANALYSIS OF VOLTAGE-SENSING MECHANISMS. Baron Chanda

1219-S ymp 4:30 pm STRUCTURAL INVESTIGATION OF A BACTERIAL VOLTAGE- GATED SODIUM CHANNEL NA V RH. Nieng Yan , Xu Zhang 1220-S ymp 5:00 pm VOLTAGE-SENSOR DOMAIN PROTEINS: PHOSPHOINOSITIDE SIGNAL, PROTON PERMEATION AND MOLECULAR TOOLS. Yasushi Okamura 1221-S ymp 5:30 pm STRUCTURAL BASIS OF VOLTAGE-DEPENDENT GATING IN CI-VSP. Eduardo Perozo , Qufei Li, Sherry Wanderlin

4:00 pm –6:00 pm , R oom 130/131 Platform Calcium Signaling

Co-Chairs Martin Falcke, Max Delbrück Center, Germany Israel Sekler, Ben Gurion University, Israel 1222-P lat 4:00 pm

RELIABLE ENCODING OF STIMULUS INTENSITIES BY RANDOM SEQUENCES OF CA 2+ SPIKES. Martin Falcke 1223-P lat 4:15 pm COUPLING OF CHEMICAL AND MECHANICAL SENSING IN FIBROBLAST CELLS. Josephine Lembong , Bo Sun, Matthew Rogers, Howard A. Stone 1224-P lat 4:30 pm NA + CHANNELS CONTROL METABOLISM AND GLOBAL CA 2+ SIGNALING BY INDUCING NA + AND CA 2+ RESPONSES THAT ARE PROPAGATING INTO THE MITOCHONDRIA OF BETA CELLS. Israel Sekler 1225-P lat 4:45 pm THE POTENTIAL FOR ANOTHER CALCIUM UPTAKE MODE IN CARDIAC MITOCHONDRIA. Christoph A. Blomeyer , Jason N. Bazil, David F. Stowe, Ranjan K. Dash, Amadou K. Camara 1226-P lat 5:00 pm ULTRAFAST GENETICALLY ENCODED CALCIUM INDICATORS FOR VISUALIZING CALCIUM FLUX AND ACTION POTENTIALS. Nordine Helassa , Elric Esposito, Tom Carter, Jonathan Bradley, David Ogden, Katalin Török 1227-P lat 5:15 pm A NEW CA 2+ PROBE, CALSTABI-CAM, TARGETED TO RYANODINE RECEPTORS OF CARDIOMYOCYTES. Sara Pahlavan , Yuming Yang, Caitlin Robertson, Naohiro Yamaguchi, Lars Cleemann, Martin Morad 1228-P lat 5:30 pm CAMKII-MEDIATED AMPLIFICATION IS ESSENTIAL TO NAADP SIGNALLING IN CARDIAC MYOCYTES. Rebecca A. Bayliss , Wee Lin, Emma Bolton, Duncan Bloor-Young, Grant C. Churchill, Antony Galione, Derek A. Terrar 1229-P lat 5:45 pm CALCIUM SIGNALING INSIDE CILIA UPON MECHANICAL BENDING. Steven Su, Siew Cheng Phua, Robert DeRose, Takanari Inoue

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Biophysical Society 58 th Annual Meeting, San Francisco, California