Single-Cell Biophysics: Measurement, Modulation, and Modeling

Single-Cell Biophysics: Measurement, Modulation, and Modeling

Poster Abstracts

101-POS Board 51 Stretching Single Native Chromain Fiber in Nanofluidic Channel for Detection of Epigenetics Marks Jia-Wei Yeh 2,1 , Kylan Szeto 2 . 1 Academia Sinica, Taipei, Taiwan, 2 Cornell University, Ithaca, NY, USA. Stretching single chromosomal DNA fibers in nanofluidic devices has become a valuable tool for studying the genome and epigenetics. Although nanofluidic technology has been extensively used in single molecular DNA analysis applications, compared to bare DNA, much less work has been done to elongate and analyze native eukaryotic chromatin fibers. Here, we provide a method for stretching and imaging individual chromatin fibers. A micro- and nanofluidic device was used to electrophoretically stretch and image single native chromatin fibers attached to microspheres held at the entrance of a nanoslit. Chromatin fragments extracted from human cancer cells (HeLa cells) were stretched and held in nanoslits, and the histone modification H3k79me2 was optically detected by fluorescence microscopy.

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