Single-Cell Biophysics: Measurement, Modulation, and Modeling

Single-Cell Biophysics: Measurement, Modulation, and Modeling

Poster Abstracts

40-POS Board 20 Irritating Effects of Sodium Lauryl Sulfate on Human Primary Keratinocytes at Sub-Toxic Level Exposure Kyung Sook Kim 1,1,1 , Hyeongwon Choi 3,3,3 , Min Kyung Shin 2,2,2 . 1 Kyung Hee University, Seoul, Seoul, South Korea, 2 Kyung Hee Unoversity, Seoul, South Korea, 3 Kyung Hee University, Seoul, South Korea. Chemical agents that can potentially cause skin irritation are tested in vivo in an animal and in vitro by cell viability or cytokine expression. These methods do not always provide translatable results and are not sensitive enough to sub-toxicity detection. In this work, we introduce the mechanical properties of keratinocytes as novel endpoints for safety assessment of chemical agents in sub-toxicity level. Human primary keratinocytes were treated with various concentrations of sodium lauryl sulfate (SLS). The response of keratinocytes to SLS was investigated by both biological and biomechanical methods. Cell proliferation, membrane integrity, inflammatory response, and cell morphology were observed using biological methods. Changes in stiffness and surface roughness as biomechanical parameters were investigated by atomic force microscopy. Morphophysiological changes were clearly seen at a relatively high dose of SLS (≥ 25 μM), while the mechanical properties of keratinocytes responded linearly to SLS at lower doses (≤ 10 μM).

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