Single-Cell Biophysics: Measurement, Modulation, and Modeling

Single-Cell Biophysics: Measurement, Modulation, and Modeling

Poster Abstracts

68-POS Board 34 Migration of Densely Packed T Cells in Microchannel HyoungJun Park 1 , HyeMi Kim 3 , Junsang Doh 1,2 . 1 POSTECH, Pohang, Kyung-buk, South Korea, 2 POSTECH, Pohang, Kyung-buk, South Korea, 3 POSTECH, Pohang, Kyung-buk, South Korea. T cell antigen recognition is a key initial step for the antigen-specific immune responses. T cell antigen recognition occurs in secondary lymphoid organs such as spleens and lymph nodes. In the lymph node, there are T cell regions where densely packed T cells continuously migrate to survey antigens presented by dendritic cells. The movement of T cells in the T cell region is important for efficient antigen recognition, but the migration of T cells in such densely packed microenvironments has not been studied so far. To study the migration of densely packed T cells, we fabricated a microchannel system filled with high density T cells. Migration of T cells within the microchannels was quantitatively analyzed by particle imaging velocimetry (PIV) methods, and further processed to extract various motility parameters, including mean velocity, vorticity, and order parameter. Roles of cytoskeletons and membrane tension were assessed by treating with pharmacological inhibitors and regulating tonicity, respectively. In addition, computer simulation was performed to further understand mechanisms of T cell motility in densely packed microenvironments.

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