Single-Cell Biophysics: Measurement, Modulation, and Modeling

Single-Cell Biophysics: Measurement, Modulation, and Modeling

Monday Speaker Abstracts

Microfluidic Single Cell Sequencing Yanyi Huang . Peking University, Beijing, China.

Biosystems are intrinsically heterogeneous. Conventional ensemble measurements heavily rely on ‘average’ values from multiple experimental replicates to study the quantitative difference between samples, or between conditions. However, the heterogeneity at the cellular level make such strategy impossible in many cases. Next generation sequencing technologies allow us to obtain large and informative data-set with relatively low cost and high throughput, and become the key driving force to facilitate our understanding of complex biosystems. However, prevalent methods are still problematic when handle highly heterogeneous samples because of the mix of real biological variations and technical variations or noises. I am going to present recent technology developments in my group, mostly the combination usage of microscopic imaging with microfluidics, to facilitate the approaches using next generation sequencing tools to analyze small number of cells or even single cells.

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