Single-Cell Biophysics: Measurement, Modulation, and Modeling

Single-Cell Biophysics: Measurement, Modulation, and Modeling

Poster Abstracts

39-POS Board 20 3D Multi-Layered Blood Vessel/Inflamed Tissue Model for the Investigation of T Cell Tissue Infiltration HyeMi Kim 3 , Sang Min Park 1 , Kwang Hoon Song 1 , Seongsu Eom 1 , HyoungJun Park 1 , Dong Sung Kim 1 , Junsang Doh 1,2 . 1 POSTECH, Pohang, South Korea, 2 POSTECH, Pohang, South Korea, 3 POSTECH, Pohang, South Korea. Leukocyte infiltration, which plays critical roles in tissue inflammation for pathogen clearance and tumor eradication, is regulated by complex microenvironments in blood vessels, including inflamed endothelium, blood flow, and perivascular components. However, the role of perivascular components on the leukocyte infiltration has not been systematically investigated until recently. In this work, we developed a 3D multi-layered blood vessel/tissue model with a nanofiber membrane, enabling real-time visualization of dynamic T cell infiltration and subsequent interaction with perivascular macrophages. We directly fabricate a highly aligned, free-standing nanofiber membrane with an ultra-thin thickness of ~ 1 μm in a microfluidic system. Coating the nanofiber membrane with matrigel showed synergetic topographical and biochemical effects on the reconstitution of a well-aligned endothelial monolayer on the membrane, which is found in native blood vessels. Fine transparency of the ultra-thin membrane allowed real-time visualization of T cells that were flowing on endothelial layers, underwent transendothelial migration and further interacted with perivascular macrophages underneath the nanofiber membrane. Our 3D multi-layered blood vessel/tissue model will offer a powerful and versatile tool to investigate the mechanism of T cell tissue infiltration.

64