Single-Cell Biophysics: Measurement, Modulation, and Modeling

Single-Cell Biophysics: Measurement, Modulation, and Modeling

Poster Abstracts

85-POS Board 43 Probing Cell-Wall Synthetic Dynamic Using Bacterial Membrane Protein-Complex Yi-Ren Sun , Chien-Jung Lo. National Central University, Chung-Li, Taiwan. Self-Replication is one of most amazing process of living organism. Bacteria have to elongate their cell body as the preparation for division. Bacterial cell-wall is made by peptidoglycan (PG). As cell elongate, elongasome, a group of membrane associate enzymes, carry out the PG insertion on the cell-wall which is guided by the cytoskeletal protein MreB filament. We aim to understand the cell-wall synthetic dynamic as cells reproduce by observing the interaction among the PG insertion, elongasome/divisome and MreB/Ftsz filaments. Bacterial flagellar motor is a protein-complex anchored on the cell membrane to rotate the extracellular flagellum for swimming. We use bacterial flagellar motor as markers to study the PG insertion. That is, when the new PG strand been inserted, the original cell-wall with the motors embedded in it will be pushed away from its original location. Therefore, by observe the movement of the motors as cells reproduce, we can observe the spatiotemporal coordination of the PG insertion. In our experiment, we observe the dynamics of fluorescent hook of flagella during the duplication process. We will discuss the implication of our observation.

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