Single-Cell Biophysics: Measurement, Modulation, and Modeling

Single-Cell Biophysics: Measurement, Modulation, and Modeling

Poster Abstracts

87-POS Board 44 Observing the Dynamics of Actin in Live Breast Cancer Cells Fang Tian 1 , Jing Li 1 , Sidong Chen 2 , Hykeun Park 1,2 . 2 the Hong Kong University of Science and Technology, Hong Kong, Hong Kong. 1 the Hong Kong University of Science and Technology, Hong Kong, Hong Kong, Actin filaments play an important role in maintaining cell morphology and dynamic processes including movement. In particular, the dynamics of actin is a key component in movement of cancer cells during metastasis. However, the dynamics of actin in live cancer cells is not clearly understood yet. We imaged actin filaments using Lifeact-GFP (a novel marker for actin filaments) in live MCF7 cells (breast cancer cells). We observed the dynamics of actin filaments during the movement of MCF7 cells. We also measured different dynamics of actin filaments under different forces in cancer cell, which were generated by a magnetic tweezers. Our studies demonstrate the dynamics of actin filaments in live cancer cells and provide a possible mechanism for metastasis of breast cancer cells to different tissues.

88