Spatial Organization of Biological Fuctions | BPS Thematic Meeting

Spatial Organization of Biological Functions Meeting

Wednesday Speaker Abstracts

MULTIPLEXED SUPERRESOLUTION IMAGING OF THE CHROMATIN LANDSCAPE REVEALS TRANSCRIPTION-DEPENDENT REORGANIZATION OF THE NUCLEAR INTERACTOME Abhinav Banerjee 1 ; Micky Anand 1 ; Mansi Srivastava 1 ; Vedanth S Vidwath 1 ; Mahipal Ganji 1 ; 1 Indian Institute of Science, Dept. of Biochemistry, Bangalore, India The spatial organization of chromatin and its associated nuclear bodies plays a central role in defining the functional state of a cell. To comprehensively map this nuclear interactome at high spatial resolution, we have developed a novel set of high-speed DNA-PAINT probes. This system extends the ability of DNA-PAINT to simultaneous image up to 10 nuclear targets with exceptional resolution and throughput. Using this platform, we investigated how transcriptional activity influences chromatin architecture and nuclear organization. By employing a DNA intercalating agent to inhibit actively transcribing RNA polymerases, we uncovered striking spatial rearrangements in key nuclear components. Specifically, we observed internalization of nuclear lamina markers, dissociation of transcriptionally active chromatin from nuclear speckles, and a widespread collapse of organized chromatin domains. These results underscore the pivotal role of transcription in maintaining nuclear architecture. Our tool provides a scalable and accessible framework to profile the nuclear interactome in diverse cellular contexts. It opens new avenues to explore cellular heterogeneity, functional cell states, and the molecular underpinnings of nuclear organization in health and disease.

ACTIVATING EPITHELIA Madan Rao National Centre for Biological Sciences, India No Abstract

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