Spatial Organization of Biological Fuctions | BPS Thematic Meeting

Spatial Organization of Biological Functions Meeting

Poster Abstracts

5-POS Board 5 INTEGRIN-DEPENDENT SPATIOTEMPORAL REGULATION OF CELL ADHESION AND CYTOSKELETAL REMODELLING DURING CELL DELAMINATION Anupriya Garg 1 ; Maithreyi Narasimha 1 ; 1 Tata Institute of Fundamental Research, Department of Biological Sciences, Mumbai, India The sculpting of tissues during morphogenesis relies on the spatiotemporal coordination of cell behaviors. The stereotypical contraction of the amnioserosa, the major force contributor for Drosophila dorsal closure, is driven by the apical constriction of the amnioserosa cells which exhibits heterogeneity in space and time. A small fraction of amnioserosa cells constrict rapidly, are extruded basally from the epithelial sheet, and change the shapes of their nearest neighbors as they do so to form a rosette. Although rare and seemingly stochastic in space and time, cell delamination contributes a third of the force generated by the amnioserosa. Work from our lab has shown that cell delamination depends on cell interactions between the delaminating cell and its neighbors, and is accompanied by the reorganization of cell adhesion and the actin cytoskeleton whose nature and regulation remain poorly understood. Using a combination of high-resolution real time confocal microscopy, quantitative image analysis and genetic perturbations, we characterize the spatiotemporal changes in cell morphology, adhesion, cytoskeletal organization and tension at the cellular interfaces during native delamination and uncover their dependence on integrin-mediated cell substrate adhesion. Our ongoing investigations aim to understand the mechanistic basis of their influence.

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