Understanding Periperal Membrane Protein Interactions | BPS Thematic Meeting

Understanding Peripheral Membrane Protein Interactions: Structure, Dynamics, Function and Therapy

Thursday Speaker Abstracts

PREDICTION OF A. THALIANA’S MCTP STRUCTURE, EMBEDDING IN THE ENDOPLASMIC RETICULUM AND INTERACTION WITH THE PLASMA MEMBRANE Sujith Sritharan 1 ; Emmanuelle Bayer 2 ; Antoine Taly ; 1 Université Paris Cité, Laboratoire de Biochimie Théorique, Paris, France 2 Université de Bordeaux, Laboratoire de Biogenèse Membranaire, Bordeaux, France In plants, intercellular communication takes place mainly via plasmodesmata, which penetrate the cell wall and create continuity between cells. The plasmodesmata are traversed by a “tube” of endoplasmic reticulum (ER), in close contact with the plasma membrane (PM), thus delimiting the pores. The family of proteins, multiple C2 domains and transmembrane region (MCTP), act as tethering proteins between the ER and the PM, specifically at the plasmodesmata.However, the mechanism of interaction with membranes and the three-dimensional structure of MCTP proteins are unknown. Thus, we generated structural models of MCTP in Arabidopsis thaliana using deep learning-based prediction methods, with a consensus approach, to create diverse starting points for coarse-grained molecular dynamics (MD) simulations using the MARTINI3 force field. The movement of transmembrane regions within the lipid bilayer of the endoplasmic reticulum was characterized by DM simulations. We then studied the interactions between C2 domains and the plasma membrane. We extracted representative conformations and identified important interactions with negatively charged PI4P lipids. Finally, we identified and characterized calcium-binding pockets in MCTP proteins using all-atom MD simulations.

MEMBRANES AS ALLOSTERIC EFFECTORS FOR LIPID TRANSFER PROTEINS Nathalie Reuter 1 ; 1 University of Bergen, Bergen, Norway

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