BPS Program Book 2014

8:00 am –5:30 pm , R oom 300 Career Center 8:00 am –6:00 pm , M arriott M arquis , P acific H, I, J Child Care 8:00 am –6:00 pm , R otunda , 300 L evel Undergraduate Student Lounge

Monday, February 17 7:30 am –8:30 am , R oom 302 Graduate Student Breakfast

This breakfast presents an opportunity for graduate student members of the Society to meet and discuss the issues they face in their current career stage. Members of the Early Careers Committee will be there to talk about grant activities specific to graduate students. They will also be available to answer questions about how the Committee serves graduate students in the biophysical community, and to recruit new Committee members. Limited to the first 100 attendees. 7:30 am –5:00 pm , N orth L obby Registration/Exhibitor Registration 7:30 am –10:00 pm , R oom 112 Family Room 8:00 am –8:45 am , R oom 123 Exhibitor Presentation FEI Company Making Correlative Experiments Easier Fluorescence microscopy excels at labeling components of the cellular machinery with unmatched sensitivity and specificity; however, it lacks any contextual information. Providing full morphological information at the ultra-structural level is the strength of electron microscopy. If the very same sample is imaged by fluorescence and electron microscopy it is possible to merge dynamics, label specificity and nanometer resolution. Although a powerful approach, it is challenging and low-throughput. FEI has recently introduced new solutions to overcome these experimental hurdles: CorrSight, a dedicated light microscopy system offering CLEM- specific functionality and automation of important workflow steps; MAPS, a software tool bridging the modalities to increase ease of use; and iCorr, a light microscope module integrated into the Tecnai family of transmission electron microscopes. These tools address different correlative workflows helping to op- timize efficiency and data quality across the full range of CLEM experiments. CorrSight is an innovative light microscope providing unprecedent solu- tions to optimal sample support for different workflows in correlative light and electron microscopy. One of its strengths is the possibility to perform live cell imaging, event-triggered fixation and subsequent processing of the sample for electron microscopy. On top of it a dedicated cryo stage allows contamination-free imaging of vitrified samples with the highest resolution. MAPS also allows for correlation of light microscopy data captured on any light microscope with EM data acquisition on the full range of FEI SEMs / SDBs. To allow utmost flexibility in the choice of the light microscope, there is absolutely no dependence on any special hardware – correlation is carried out only on image data. Thus, existing or specialized light micros- copy setups can be easily used for CLEM experiments and correlation can be carried out on any feature visible in both modalities. When coupled with CorrSight the correlation is possible without manual intervention. In order to perform correlative experiments between LM and TEM imag- ing FEI has developed an integrated light and electron microscope: iCorr. This tool provides fast and effortless navigation for correlative experiments. Presenters: Alex de Marco, Product Marketing Manager, FEI Munich GmbH Gregor Heiss, Product Marketing Engineer, FEI Munich GmbH Liesbeth Hekking, Applications Development Engineer, FEI Company Matthias Langhorst, Segment Director Cell Biology Solutions, FEI Company

This special space is reserved for undergraduate meeting attendees looking for a place to relax or catch up on coursework they may be missing while at the Annual Meeting. Members of the Education Committee, which sponsors this lounge, will stop by to answer questions student attendees may have about career paths and opportunities. 8:00 am –10:00 pm , H all D Poster Viewing 8:15 am –10:15 am , R oom 134 Symposium Molecular Basis for Regulation of Ca 2+ Channels Co-Chairs Amy Lee, University of Iowa Stephen Long, Memorial Sloan-Kettering Cancer Center 1111-S ymp 8:15 am ORIGIN AND MECHANISM OF MITOCHONDRIAL FLASHES. Heping Cheng , Xianhua Wang, Qi Ma, Wang Wang 1112-S ymp 8:45 am CAV1.3 L-TYPE CALCIUM CHANNEL DYSFUNCTION IN HU- MAN DISEASE. Jörg Striessnig 1113-S ymp 9:15 am REGULATION OF VOLTAGE-GATED CALCIUM CHANNEL TRAFFICKING AND FUNCTION BY AUXILIARY SUBUNITS. Annette C. Dolphin 1114-S ymp 9:45 am 3D STRUCTURES OF THE CALCIUM RELEASE-ACTIVATED CALCIUM CHANNEL ORAI. Stephen B. Long

8:15 am –10:15 am , R oom 135 Symposium Force Sensing in Muscle

Co-Chairs Mathias Gautel, King’s College London, United Kingdom Gabriella Piazzesi, University of Florence, Italy 1115-S ymp 8:15 am THE ELASTICITY OFTHE MYOSINMOTOR AND MYOFILAMENTS IN THE MUSCLE SARCOMERE.

Gabriella Piazzesi, Luca Fusi, Marco Caremani, Elisabetta Brunello, Massimo Reconditi, Luca Melli, Marco Linari, Malcolm Irving, Vincenzo Lombardi 1116-S ymp 8:45 am EXPERIMENTAL AND COMPUTATIONAL APPROACHES TO STUDY MYOFILAMENT STRUCTURE-FUNCTION IN NORMAL AND DISEASED MUSCLE. Michael Regnier, Yuanhua Cheng, Pete Kekenes-Huskey, Steffen Lindert, Andrew McCulloch

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Biophysical Society 58 th Annual Meeting, San Francisco, California