BPS2024 Full Program & Abstracts

3:30 pm – 5:00 pm LUMICKS

lation of a 1.2 GHz spectrometer on the North American continent at Ohio State University and the installation of 1.1 GHz instruments at the University of Wisconsin at Madison and the University of Georgia. Bruker BioSpin has also introduced the HelioSmart series of Helium recovery and liquification systems to reduce helium consumption for ultra-high field magnets. On the data processing and analysis end, Bruker BioSpin has introduced deep learning-based modules in their software for better data processing and peak analysis and is collaborat ing with academic labs on the implementation of AI and machine learn ing in the analysis of NMR data. The current status of these collabora tions will be presented. In summary, both EPR and NMR are powerful techniques for biophysi cal research, and Bruker BioSpin is at the forefront of developing and introducing novel products to facilitate research. From ultra-high field NMR magnets and helium recovery systems to deep learning-based modules in their software, Bruker BioSpin is committed to advancing the field of biophysical research. Speakers Clemens Anklin, Vice President, NMR Applications & Training, Bruker BioSpin Alvaro Montoya, EPR Application Scientist, Bruker BioSpin 2:30 pm – 4:00 pm Thermo Fisher Scientific Achieving Cryo-EM Solutions with 100kV Data Collection To fully understand biological processes, and how they fail in disease, it is vital to obtain structural information for the relevant biological machinery. Notably, it is becoming increasingly apparent that proteins, the key biological players in fundamental biology or disease mecha nisms, often adopt multiple conformations or act in complexes with other proteins. These large and/or dynamic systems present a challenge to traditional methods of 3D structural determination as X-ray crystallography or NMR. Fortunately, cryo-electron microscopy (Cryo-EM) techniques, particularly single particle analysis (SPA), have emerged as a well-suited approach for the determination of native protein function, the dynam ics of complex biological systems, and drug discovery applications. In Cryo-EM, specimens are rapidly frozen (vitrified) so that their biologi cally relevant native states are preserved. SPA can then obtain struc tural details of the specimen at near-atomic resolution. This technique has transformed the field of structural biology, leading to new insight into numerous biological processes. During this seminar, Dr. Natalia de Val will talk about how cryo-EM is revolutionizing structural biology, and she will present the capabilities of our cryo-transmission electron microscope Tundra coupled with our newest Direct Electron Detector (DED) camera, Falcon-C. Our Tundra cryo-TEM is dedicated to SPA, bringing this powerful tech nique to every biochemistry laboratory. The Tundra Cryo-TEM is spe cially designed for new users who are interested in adopting electron microscopy but may not possess expertise in the field. It is easier to use than typical cryo-TEM instruments, fits into a standard lab space, and matches funding opportunities globally. The Tundra, combined with our new DED for 100kV, Falcon-C, is a powerful tool that can help answer your most challenging research questions, offering structural determination at biologically relevant resolutions. Speaker Natalia de Val, Senior Scientist, Electron Microscopy, Thermo Fisher Scientific

Accelerating Biological Discovery: An Automated, End-to-End Solu tion in Single-Molecule Research Dynamic single-molecule research has revolutionized our understand ing of biology by providing crucial insights into key molecular and cel lular mechanisms, such as DNA repair and phase separation processes, among others. However, this field of science has traditionally been accessible only to experts capable of building and operating complex home-built instru ments, able to produce specific DNA or protein samples, and develop ing sophisticated algorithms for correct single-molecule data analysis and interpretation. To democratize access to single-molecule technologies, LUMICKS C-Trap was introduced as the first instrument of its kind. LUMICKS C-Trap uniquely combines optical tweezers, single-molecule fluores cence, and automated microfluidics, allowing the correlation of single molecule force and fluorescence measurements in real time to enable the investigation of dynamic molecular mechanisms with a ready-to-go instrument. Here, we will unveil our latest advancements in creating a comprehen sive end-to-end solution designed to transform the field of dynamic single-molecule research: from a vast range of DNA and protein samples to the full automation for instrument set-up and experiment execution, all the way to a seamless data analysis and interpretation. Speaker Trey Simpson, Senior Application Scientist, LUMICKS Room 113C: Sunday, February 11 10:30 am – 12:00 pm Bruker Applications of Electron Paramagnetic Resonance (EPR) and Nuclear Magnetic Resonance (NMR) in Biophysical Research Paramagnetic species and free electrons are common in biological sys tems, and Electron Paramagnetic Resonance (EPR) is the best method for detecting metals and free radicals in biological samples. EPR pro vides information on the physical and electronic structure around a paramagnetic center and can be performed in both continuous wave (CW) and Pulse formats. CW EPR provides general information on the spin center and its surroundings, while Pulse EPR offers detailed knowl edge of primary to quaternary structure. Hyperfine Spectroscopies, such as Pulse-ENDOR and HYSCORE, provide information on the hyper fine interaction and can map enzyme active sites, binding interactions, and local changes during catalysis. Site-Directed Spin Labeling (SDSL) introduces unpaired spins into proteins, enzymes, or nucleotides, and Pulsed Dipolar Spectroscopy (PDS) measures the dipolar coupling between two spins to gain dynamic information on a larger scale. PDS can yield distance distributions, providing information on the dynamics of regions of interest and temporal resolution of the ensemble. Shaped pulses using Arbitrary Waveform Generator (AWG) technology have opened new avenues for researchers to improve existing experiments and design new schemes for PDS. Nuclear Magnetic Resonance (NMR) is another powerful technique for biophysical research, and Bruker BioSpin has introduced novel products to facilitate research. Bruker BioSpin continues to drive the evolution of ultra high field NMR magnets, with the completion of the first instal

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