BPS2024 Full Program & Abstracts

Room 113C: Monday, February 12 10:30 am – 12:00 pm HORIBA Scientific

2:30 pm – 4:00 pm PicoQuant Photonics North America Inc Making Confocal Fluorescence Microscopy a Tool for Every Biophysicist Quantitative time-resolved fluorescence techniques like FLIM, FCS and single molecule FRET (smFRET) are increasingly employed in biophysical research in fields like dynamic structural biology, mechanisms driven by phase separation and cellular environment sensing. PicoQuant`s new confocal microscope Luminosa combines state-of-the art hardware with cutting edge software to deliver high quality data while simplifying daily operation. The software includes several fea tures including context-based workflows, sample-free auto-alignment and excitation laser power calibration which improve the reproduc ibility of experiments. Still, if required for method development every We will show how Luminosa brings smFRET to a new level. For example, FRET efficiency (E) and stoichiometry (S) are calculated online, corrected according to the standard procedure of the com munity, and displayed live in an E/S histogram during the mea surement. In addition, the ability to vary the detection volume with a single click of a button will give researchers more flexibility regarding determining dynamic structural changes. • We will describe how FLIM is streamlined. It’s rapidFLIM hardware can record several frame per second with high photon count rates, which the software handles with a novel dynamic binning format. In combination with GPU-accelerated algorithms, this enables high-speed automated analysis of FLIM images with mini mal user interactions. As an outlook we will demonstrate how Luminosa can combine FLIM with super-resolution imaging modalities. Speakers Marcelle Koenig, Senior Scientist R&D, PicoQuant GmbH Evangelos Sisamakis, Product Manager Microscopy, PicoQuant GmbH Room 113C: Tuesday, February 13 10:30 am – 12:00 pm Journal of General Physiology has published exciting developments in membrane and cellular physiology for more than 100 years. For this presentation, JGP has invited three early career authors to describe the advances in their work on the subject. An introduction to the journal by JGP Editor-in-Chief David Eisner will precede the talks, and time will be provided for further discussion. Presentations include: “Lower Troponin in Rat Right Ventricle Explains Intraventricular E-C Coupling Differences,” by Young Keul Jeon, Department of Physiology, Ischemic/Hypoxic Disease Institute, Seoul National University College of Medicine, Seoul, Republic of Korea. optomechanical component can be accessed. In this talk two use cases will be presented: • Journal of General Physiology (JGP) Advances in Membrane Physiology 2024

New and Unique FLIM Solutions from HORIBA Fluorescence Division In this presentation, we will introduce new and unique fluorescence lifetime imaging (FLIM) solutions from the fluorescence division of HORIBA Instruments Inc including laser scanning confocal FLIM, to stage-scanning FLIM and our revolutionary wide field video rate FLIM video camera (FLIMera). Demonstrations are also available in our booth #518. Speaker Cary Davies, Global Product Line Manager, HORIBA Scientific 12:30 pm – 2:00 pm Leica Microsystems New TauSTED Tools for Gentle Live Cell Imaging at Remarkable Nanoscale The ultimate goal of scientific research is to understand the workings of nature. Given the complex interplay of biomolecules, molecular machines, and higher order cellular structures, confocal imaging has emerged as the fundamental tool due to its optical sectioning, sensitiv ity, and temporal and spatial resolution capabilities. Imaging intricate cellular structures at nanoscale resolution while characterizing the dynamics of multiple biomolecules in the context of live specimens is an emerging method shedding light on biological processes. In this talk, we will present how new STELLARIS STED innovations enable gentle live cell imaging at nanoscale resolution. We will show how advances on our TauSTED approach to optical nanoscopy deliver cutting-edge resolution and image quality at low light dose, key to accessing fast nanoscale dynamics of cellular processes. We will also show how fluorescence lifetime information can be used to multiplex different markers while maintaining nanoscale resolution. Finally, we will show how our STED FCS approach can measure diffusion of fluoro phores at high concentration. Speakers Giulia Ossato, Senior Product Manager, Leica Microsystems Haridas Pudavar, Product Performance Manager, Leica Microsystems M. Julia Roberti, Senior Product Manager, Leica Microsystems

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