Biophysical Society 2020 Annual Meeting

2381-Plat 8:30 am Travel Awardee INDIRECT BACTERIAL TRANSCRIPTION-TRANSLATION COUPLING MECHA- NISM REVEALED BY IN SITU INTEGRATIVE STRUCTURAL BIOLOGY.  Liang Xue , Francis O’Reilly, Ludwig Sinn, Juri Rappsilber, Julia Mahamid 2382-Plat 8:45 am THE IN SITU STRUCTURE OF PARKINSON’S DISEASE-LINKED LRRK2.  Reika Watanabe , Robert Buschauer, Jan Böhning, Martina Audagnotto, Keren Lasker, Tsan Wen Lu, Daniela Boassa, Susan S. Taylor, Elizabeth Villa 2383-Plat 9:00 am IN SITU ARRANGEMENT OF INFLUENZA A VIRUS MATRIX PROTEIN M1 RESOLVED BY CRYO ELECTRON TOMOGRAPHY SUGGESTS A MODEL FOR VIRUS ASSEMBLY.  Julia Peukes , Serge Dmitrieff, John A.G. Briggs 2384-Plat 9:15 am NMR “CRYSTALLOGRAPHY” OF MEMBRANE PROTEINS ALIGNED IN NATIVE-LIKE BILAYERS. Joel Lapin, Emmanuel Awosanya, Alexander 9:30 am AB INITIO ELECTRON DENSITY DETERMINATION DIRECTLY FROM SOLU- TION SCATTERING DATA.  Thomas D. Grant 2386-Plat 9:45 am PROTEIN CRYSTAL MOTIONS FROM TIME-RESOLVED DIFFRACTED X-RAY BLINKING.  Yuji C. Sasaki , Masahiro Kuramochi, Kazuhiro Mio, Hiroshi Sekiguchi, Ayana Sato-Tomita, Naoya Shibayama 2387-Plat 10:00 am OVERHAUSER DYNAMIC NUCLEAR POLARIZATION: A TOOL FOR BUILDING MAPS OF HYDRATION WATER.  John M. Franck Platform Exocytosis and Endocytosis 8:15 am - 10:15 am, Room 30ABC Co-Chairs Bianxiao Cui, Stanford University Sathish Thiyagarajan, Columbia University 2388-Plat 8:15 am CRYO-EM OF INTACT CLATHRIN-COATED VESICLES REVEALS ADAPTOR DISTRIBUTION AND NOVEL INTERACTIONS BETWEEN SUBUNITS.  Mohammadreza Paraan , Scott M. Stagg 2389-Plat 8:30 am NANOSCALE CURVATURES MODULATE PROTEIN SIGNALING AT THE CELL MEMBRANE.  Bianxiao Cui , Xiao Li, Wei Zhang, Lasse Klausen, Hsin-Ya Lou, Wenting Zhao 2390-Plat 8:45 am CRYO-EM STRUCTURES OF FULL-LENGTH DYNAMIN ASSEMBLED ON MEMBRANES IN VITRO AND WITHIN CELLS.  John Jimah , Abigail Stanton, Kem A. Sochacki, Lieza M. Chan, Haifeng He, Huaibin Wang, Justin W. Taraska, Jenny E. Hinshaw 2391-Plat 9:00 am DEMYSTIFYING DYNAMICS OF DYNAMIN DURING CLATHRIN MEDIATED ENDOCYTOSIS.  Ning Fang , Xiaodong Cheng, Kuangcai Chen, Bin Dong 2392-Plat 9:15 am DYNAMIN FUNCTION IN EXOCYTOSIS AND ENDOCYTOSIS COUPLING OF DENSE-CORE VESICLES IN PANCREATIC BETA CELLS.  Fan Fan, Jenifer Wendlick, Natalia Tamarina, Yumei Wu, Shawn Ferguson, Louis H. Philip- son, Pietro De Camilli, Xuelin Lou 2393-Plat 9:30 am ESCRT-III ASSEMBLES SIMULTANEOUSLY AND WITHOUT PREFERENCE ON SUPPORTED LIPID BILAYERS OF VARYING CURVATURES.  Nebojsa Jukic , Alma P. Perrino, Simon Scheuring Nevzorov 2385-Plat

2370-Plat 9:45 am DE NOVO DESIGN OF ION CONDUCTING TRANSMEMBRANE PROTEIN NANOPORES.  Sinduja K. Marx , Anastassia Vorobieva, Cameron Chow, Jonathan M. Craig, Hwanhee C. Kim, Sarah J. Abell, Jesse Huang, Stacey Gerben, David Baker, Jens H. Gundlach 2371-Plat 10:00 am EXPRESSION AND CHARACTERIZATION OF CONNEXIN30.3.  Jesse Asiedu, Ingrid M. Skerrett Platform Protein Assemblies 8:15 am - 10:15 am, Room 24ABC Co-Chairs Marcia Levitus, Arizona State University Allen Minton, NIH, NIDDK 2372-Plat 8:15 am E. COLI SINGLE-STRANDED DNA BINDING (SSB) PROTEIN UNDERGOES DY- NAMIC LIQUID-LIQUID PHASE SEPARATION CONTROLLED VIA PROTEIN- PROTEIN AND PROTEIN-DNA INTERACTIONS.  Gabor Harami , Zoltan J. Kovacs, János Pálinkás, Rita Pancsa, Veronika Baráth, Krisztián Tárnok, Hajnalka Harami-Papp, Andras Malnasi-Csizmadia, Mihaly Kovacs 2373-Plat 8:30 am DIRECT OBSERVATION OF PRION PROTEIN FIBRIL ELONGATION KINETICS.  Yuanzi Sun , Mark Batchelor, John Collinge, Jan Bieschke 2374-Plat 8:45 am MODULATION OF THE OLIGOMERIZATION STATE OF PROTEINS BY IONS AND SMALL MOLECULES. AN FCS STUDY.  Anirban Purohit, Linda B. Bloom, Marcia Levitus 2375-Plat 9:00 am SIMPLE CALCULATION OF PHASE DIAGRAMS FOR LIQUID-LIQUID PHASE TRANSITIONS IN SOLUTIONS OF TWO MACROMOLECULAR SOLUTE SPE- CIES.  Allen P. Minton 2376-Plat 9:15 am COMPUTER SIMULATIONS OF KEY PEPTIDES INVOLVED IN PREECLAMP- SIA AND ALZHEIMER’S DISEASE. Maksim Kouza , Andrzej Kolinski, Irina Buhimschi, Andrzej Kloczkowski 2377-Plat 9:30 am DETERMINING THE OLIGOMERIC STATES OF A GPI-ANCHORED MODEL PROTEIN VIA COLOCALIZATION-BASED SINGLE-MOLECULE MICROSCOPY.  Clara Bodner , Mario Brameshuber, Gerhard J. Schütz 2378-Plat 9:45 am LIQUID-LIQUID PHASE SEPARATION OF WHEAT GLIADINS - TOWARDS PHYSIOLOGICAL CONDITIONS.  Line Sahli , Denis Renard, Véronique Solé- Jamault, Adeline Boire 2379-Plat 10:00 am Travel Awardee P53 DEAMIDATION AS A MOLECULAR TIMER FOR CELL DEATH.  Karola Gerecht , Sofia Margiola, Manuel M. Müller Platform

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NMR, Diffraction, and EM 8:15 am - 10:15 pm, Room 25ABC

Co-Chairs John Franck, Syracuse University Jessica Rabuck-Gibbons, The Scripps Research Institute 2380-Plat 8:15 am

QUANTITATIVE ANALYSIS OF LATE-STAGE RIBOSOME ASSEMBLY WITH CRYO-EM.  Jessica N. Rabuck-Gibbons , Dmitry Lyumkis, James R. Wil- liamson

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